Human CANT1 Gene cDNA Clone / ORF Clone

Human CANT1 cDNA Clone Datasheet

CANT1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_138793.3
• cDNA Size: 1206
• Gene Synonym: DBQD; SCAN1; SHAPY; SCAN-1; CANT1
• Source: Human

CANT1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens calcium activated nucleotidase 1 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 5 C/A resulting in the amino acid pro substitution by his and 159 T/C not causing the amino acid variation.

CANT1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CANT1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CANT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCACGTGC AGCTGTCTGA GCACCCGGAA TGGAATGAGT CTATGCACTC CCTCCGGATC
61       AGTGTGGGGG GCCTTCCTGT GCTGGCGTCC ATGACCAAGG CCGCGGACCC CCGCTTCCGC
121      CCCCGCTGGA AGGTGATCCT GACGTTCTTT GTGGGTGCCG CCATCCTCTG GCTGCTCTGC
181      TCCCACCGCC CGGCCCCCGG CAGGCCCCCC ACCCACAATG CACACAACTG GAGGCTCGGC
241      CAGGCGCCCG CCAACTGGTA CAATGACACC TACCCCCTGT CTCCCCCACA AAGGACACCG
301      GCTGGGATTC GGTATCGAAT CGCAGTTATC GCAGACCTGG ACACAGAGTC AAGGGCCCAA
361      GAGGAAAACA CCTGGTTCAG TTACCTGAAA AAGGGCTACC TGACCCTGTC AGACAGTGGG
421      GACAAGGTGG CCGTGGAATG GGACAAAGAC CATGGGGTCC TGGAGTCCCA CCTGGCGGAG
481      AAGGGGAGAG GCATGGAGCT ATCCGACCTG ATTGTTTTCA ATGGGAAACT CTACTCCGTG
541      GATGACCGGA CGGGGGTCGT CTACCAGATC GAAGGCAGCA AAGCCGTGCC CTGGGTGATT
601      CTGTCCGACG GCGACGGCAC CGTGGAGAAA GGCTTCAAGG CCGAATGGCT GGCAGTGAAG
661      GACGAGCGTC TGTACGTGGG CGGCCTGGGC AAGGAGTGGA CGACCACTAC GGGTGATGTG
721      GTGAACGAGA ACCCGGAGTG GGTGAAGGTG GTGGGCTACA AGGGCAGCGT GGACCACGAG
781      AACTGGGTGT CCAACTACAA CGCCCTGCGG GCTGCTGCCG GCATCCAGCC GCCAGGCTAC
841      CTCATCCATG AGTCTGCCTG CTGGAGTGAC ACGCTGCAGC GCTGGTTCTT CCTGCCGCGC
901      CGCGCCAGCC AGGAGCGCTA CAGCGAGAAG GACGACGAGC GCAAGGGCGC CAACCTGCTG
961      CTGAGCGCCT CCCCTGACTT CGGCGACATC GCTGTGAGCC ACGTCGGGGC GGTGGTCCCC
1021     ACTCACGGCT TCTCGTCCTT CAAGTTCATC CCCAACACCG ACGACCAGAT CATTGTGGCC
1081     CTCAAATCCG AGGAGGACAG CGGCAGAGTC GCCTCCTACA TCATGGCCTT CACGCTGGAC
1141     GGGCGCTTCC TGTTGCCGGA GACCAAGATC GGAAGCGTGA AATACGAAGG CATCGAGTTC
1201     ATTTAA

 

Quality Control:cDNA sequencing results is available upon request.