Application | Dilution |
---|---|
WB | 1:500-1:1000 |
ELISA | 1:25000-1:50000 |
IP | 0.2-1 μL/mg of lysate |
CAMK1 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
0.5 µL anti-CAMK1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-CAMK1 rabbit polyclonal antibody,at 1:500 dilution
Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 40 kDaObserved band size: 40 kDa
Anti-CAMKII rabbit polyclonal antibody at 1:500 dilution
Lane A: Hela Whole Cell Lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:41 kDaObserved band size:45 kDa