-HB05JL0604-IP.png)
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| Application | Dilution | Notes |
|---|---|---|
| WB | 1:500-1:1000 | |
| ELISA | 1:25000-1:50000 | |
| IP | 0.2-1 μL/mg of lysate |
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CAMK1 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
0.5 µL anti-CAMK1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-CAMK1 rabbit polyclonal antibody,at 1:500 dilution
Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 40 kDaObserved band size: 40 kDa
Anti-CAMKII rabbit polyclonal antibody at 1:500 dilution
Lane A: Hela Whole Cell Lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:41 kDaObserved band size:45 kDa
Calcium/calmodulin-dependent protein kinase or CaM kinases are serine/threonine-specific protein kinases that are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. In neurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction of long-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse.
Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of the protein kinase superfamily and CaMK subfamily. It contains one protein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.
| Product Description | Host | Clonality | Application | Catalog# (PDF) |
|---|---|---|---|---|
| Anti-CAMKI Antibody | Rabbit | Monoclonal | ELISA | 11932-R010 |
