Anti-CAMKI Antibody

Cat: 11932-RP02
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All CAMKI Reagents
CRO Services
Product recommended

All CAMKI Reagents
Anti-CAMKI Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CAMKI Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human CAMKI
Immunogen
Recombinant Human CAMKI / CAMK1 protein (Catalog#11932-HNCE)
Preparation
Produced in rabbits immunized with purified, recombinant Human CAMKI / CAMK1 (rh CAMKI / CAMK1; Catalog#11932-HNCE; NP_003647.1; Leu 2-Leu 370). CAMKI / CAMK1 specific IgG was purified by Human CAMKI / CAMK1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CAMKI Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CAMKI Antibody (Rabbit Polyclonal antibody) Images
Human CAMKI/CAMK1 Immunoprecipitation(IP) 8112

CAMK1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-CAMK1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CAMK1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Human CAMKI/CAMK1 Western blot (WB) 13454

Anti-CAMKII rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:41 kDa

Observed band size:45 kDa

Anti-CAMKI Antibody Alternative Names
Anti-CAMKI Antibody
CAMKI Background Information

Calcium/calmodulin-dependent protein kinase or CaM kinases are serine/threonine-specific protein kinases that are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. In neurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction of long-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse.

Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of the protein kinase superfamily and CaMK subfamily. It contains one protein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.

Full Name
calcium/calmodulin-dependent protein kinase I
References
  • Lisman, JE. et al., 1985, Proc Natl Acad Sci USA. 82 (9): 3055-7.
  • Bergamaschi, A. et al., 2008, Mol Oncol. 2 (4): 327-39.
  • White RB. et al., 2008, Physiological genomics, 33 (1): 41-9.
  • Schleinitz, D. et al., 2010, Horm Metab Res. 42 (1): 14-22.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CAMKI Antibody Rabbit Monoclonal ELISA 11932-R010
    info info
    添加购物车成功! 添加购物车失败!