Mouse CAMK4 / CaMKIV Protein (GST Tag)

Calcium / Calmodulin-dependent protein kinase IV Protein Datasheet

CAMK4 / CaMKIV Protein Product Information

• Synonym: Camk4
• Protein Construction: A DNA sequence encoding the mouse CAMK4(P08414) (Met1-Tyr469) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

CAMK4 / CaMKIV Protein QC Testing

• Purity: > 94 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The secreted recombinant mouse CAMK4/GST chimera consists of 706 amino acids and has a calculated molecular mass of 80.4 kDa. The recombinant protein migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10% gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CAMK4 / CaMKIV protein

CAMK4 / CaMKIV Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CAMK4 / CaMKIV Protein Description

Calcium/calmodulin-dependent protein kinase type IV, also known as CaMK IV, CaM kinase-GR and CAMK4, is cytoplasm and nucleus protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CAMK4 is expressed in epithelial ovarian cancer tissue. CAMK4 is a calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. CAMK4 is a multifunctional serine/threonine protein kinase with limited tissue distribution, it has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. It may be involved in transcriptional regulation. CAMK4 may also be involved in regulation of microtubule dynamics. CAMK4 phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. It may play a role in the consolidation/retention of hippocampus-dependent long-term memory. CAMK4 is activated by Ca²⁺/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. It is phosphorylated by CAMKK1 or CAMKK2. CAMK4 interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca²⁺/calmodulin.

References

1. Chatila T. et al.,1996, J Biol Chem. 271: 21542-8.
2. Melander Gradin H. et al., 1997, Mol Cell Biol. 17: 3459-67.
3. Blaeser F. et al., 2000, J Biol Chem. 275: 197-209.
4. Anderson KA. et al., 2004, J Biol Chem. 279: 31708-16.
5. Wissing J. et al., 2007, Mol Cell Proteomics. 6: 537-47.