CAMK2A / CAMKA Protein (GST Tag)

Calcium/calmodulin-Dependent Protein Kinase Type II subunit alpha

CAMK2A Protein Product Information

• Synonym: CAMKA, KIAA0968, CAMK2A
• Protein Construction: A DNA sequence encoding the human CAMK2A isoform 2 (NP_741960.1) (Met 1-His 478) was fused with the GST tag at the N-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

CAMK2A Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CAMK2A/GST chimera consists of 703 amino acids and predicts a molecular mass of 80.3 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Tris,100mM NaCl, 0.5mM PMSF,0.5mM Reduced Glutathione,pH 8.0
• SDS-PAGE: CAMK2A protein

CAMK2A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

CAMK2A Protein Description

Calcium/calmodulin-dependent protein kinase type I I subunit alpha, also known as CaM kinase II subunit alpha, CAMKA, and CAMK2A, is a member of the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2A contains one protein kinase domain. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. As a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CAMK2 interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1.

References

1. Nagase T., et al., 1999, DNA Res. 6: 63-70.
2. Schmutz J., et al., 2004, Nature. 431: 268-274.
3. Krapivinsky G., et al., 2004, Neuron 43:563-574. 
4. Ignotz,GG. et al., 2005, Biol Reprod. 73 (3): 519-26.