CAMK1G / CLICK III / CaMKIgamma Protein (GST Tag)

Calcium/calmodulin-dependent protein kinase IG Protein Datasheet

CAMK1G / CLICK III / CaMKIgamma Protein Product Information

• Synonym: RP1-272L16.2, CLICKIII, VWS1, dJ272L16.1
• Protein Construction: A DNA sequence encoding the human CAMK1G isoform 1 (?Q96NX5-1) (Met 1-Met 476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

CAMK1G / CLICK III / CaMKIgamma Protein QC Testing

• Purity: >85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CAMK1G/GST chimera consists of 713 amino acids and has a calculated molecular mass of 81KDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH8.0, 20% gly, 0.3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CAMK1G / CLICK III / CaMKIgamma protein

CAMK1G / CLICK III / CaMKIgamma Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CAMK1G / CLICK III / CaMKIgamma Protein Description

Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases (CaMKs). Calmodulin-dependent protein kinase CL3 (CLICK III)/CaMKIγ is a memberane-anchored CaM kinase belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIγ membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIγ critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIγ specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIγ may represent a key element in the Ca(²⁺)-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.

References

1. Davare MA. et al., 2009, J Neurosci. 29 (31): 9794-808.
2. Takemoto-Kimura S. et al., 2007, Neuron. 54 (5): 755-70.
3. Harrill JA. et al., 2010, J Biochem Mol Toxicol. 24 (3):174-86.