All CaMKI gamma reagents are produced in house and quality controlled, including 1 CaMKI gamma Gene, 1 CaMKI gamma Lysate, 1 CaMKI gamma Protein. All CaMKI gamma reagents are ready to use.
Recombinant CaMKI gamma proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-GST & His.
CaMKI gamma cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each CaMKI gamma of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIγ is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIγ membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIγ critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIγ specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIγ may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.
CaMKI gamma References
Davare MA, et al. (2009) Transient receptor potential canonical 5 channels activate Ca2+ / calmodulin kinase Igamma to promote axon formation in hippocampal neurons. J Neurosci. 29 (31): 9794-808.
Takemoto-Kimura S, et al. (2007) Regulation of dendritogenesis via a lipid-raft-associated Ca2+ / calmodulin - dependent protein kinase CLICK-III / CaMKIgamma. Neuron. 54 (5): 755-70.
Harrill JA, et al. (2010) Splice variant specific increase in Ca2+ / calmodulin-dependent protein kinase 1-gamma mRNA expression in response to acute pyrethroid exposure. J Biochem Mol Toxicol. 24 (3): 174-86.