CALML3 Protein (His Tag)

Calmodulin-like 3 Protein Datasheet

CALML3 Protein Product Information

• Synonym: CLP
• Protein Construction: A DNA sequence encoding the human CALML3 (P27482) (Met 1-Lys 149) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

CALML3 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CALML3 consisting of 164 amino acids and has a calculated molecular mass of 18.7KDa. It migrates as an 16 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CALML3 protein

CALML3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CALML3 Protein Description

Calmodulin-like protein 3 (CALML3) is similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinity, to cellular substrates. Calmodulin-like protein 3 (CALML3) is a tumor-sensitive protein specifically expressed in normal epithelial cells but downregulated in tumorigenesis. Downregulation of the protein is an early event in breast cancer development. One of the most pressing questions raised by the discovery of CLP/CALML3 is that of its potential targets. Although it is 85% identical to human calmodulin, the distinct properties of CLP suggest that it has specific targets or targets that only partially overlap with those of calmodulin. Fluorescence microscopy and Western blotting revealed that CLP expression results in up-regulation of its target protein, myosin-10. This up-regulation occurs at the protein level by stabilization of myosin-10. Thus, CLP functions by increasing the stability of myosin-10, leading to enhanced myosin-10 function and a subsequent increase in filopodial dynamics and cell migration.

References

1. Bennett RD. et al., 2007, J Biol Chem. 282 (5): 3205-12.
2. Perochon A. et al., 2011, Biochimie. 93 (12): 2048-53.
3. Chinpongpanich A. et al., 2011, Acta Biochim Biophys Sin (Shanghai). 43 (11): 867-76.