Human CALCB Gene cDNA Clone / ORF Clone

CALCB cDNA Clone Datasheet

CALCB cDNA Clone Product Information

• Gene Bank Ref.ID: BC008428
• cDNA Size: 384
• Gene Synonym: CALC2; CGRP2; CGRP-II; CALCB
• Source: Human

CALCB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens calcitonin-related polypeptide beta DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CALCB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CALCB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CALCB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTTTCC GGAAGTTCTC CCCCTTCCTG GCTCTCAGTA TCTTGGTCCT GTACCAGGCG
61       GGCAGCCTCC AGGCGGCGCC ATTCAGGTCT GCCCTGGAGA GCAGCCCAGA CCCGGCCACA
121      CTCAGTAAAG AGGACGCGCG CCTCCTGCTG GCTGCACTGG TGCAGGACTA TGTGCAGATG
181      AAGGCCAGTG AGCTGAAGCA GGAGCAGGAG ACACAGGGCT CCAGCTCCGC TGCCCAGAAG
241      AGAGCCTGCA ACACTGCCAC CTGTGTGACT CATCGGCTGG CAGGCTTGCT GAGCAGATCA
301      GGGGGCATGG TGAAGAGCAA CTTCGTGCCC ACCAATGTGG GTTCCAAAGC CTTTGGCAGG
361      CGCCGCAGGG ACCTTCAAGC CTGA

 

Quality Control:cDNA sequencing results is available upon request.