Human CALCA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13235-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CALCA cDNA Clone Datasheet

CALCA cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001033952.2
cDNA Size:	426
Gene Synonym:	CT; KC; CGRP; CALC1; CGRP1; CGRP-I; MGC126648; CALCA
Source:	Human

CALCA cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens calcitonin-related polypeptide alpha DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CALCA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CALCA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CALCA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTTCC AAAAGTTCTC CCCCTTCCTG GCTCTCAGCA TCTTGGTCCT GTTGCAGGCA
61       GGCAGCCTCC ATGCAGCACC ATTCAGGTCT GCCCTGGAGA GCAGCCCAGC AGACCCGGCC
121      ACGCTCAGTG AGGACGAAGC GCGCCTCCTG CTGGCTGCAC TGGTGCAGGA CTATGTGCAG
181      ATGAAGGCCA GTGAGCTGGA GCAGGAGCAA GAGAGAGAGG GCTCCAGCCT GGACAGCCCC
241      AGATCTAAGC GGTGCGGTAA TCTGAGTACT TGCATGCTGG GCACATACAC GCAGGACTTC
301      AACAAGTTTC ACACGTTCCC CCAAACTGCA ATTGGGGTTG GAGCACCTGG AAAGAAAAGG
361      GATATGTCCA GCGACTTGGA GAGAGACCAT CGCCCTCATG TTAGCATGCC CCAGAATGCC
421      AACTAA

Quality Control:cDNA sequencing results is available upon request.