Rat CADM3 Gene cDNA Clone / ORF Clone

CADM3 cDNA Clone Datasheet

CADM3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001047103.1
• cDNA Size: 1191
• Gene Synonym: Igsf4b, Necl-1, Cadm3
• Source: Rat

CADM3 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus cell adhesion molecule 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 492 A/G not causing the amino acid variation.

CADM3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CADM3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CADM3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGCCC CTTCCGCCCT GCCCCTGCTC CTGCTCCTCG CCTGCTCCTG GGCGCCCGGC
61       GGGGCCAATC TTTCCCAGGA CGATAGCCAG CCCTGGACGT CTGATGAAAC AGTGGTGGCT
121      GGTGGCACAG TAGTGCTCAA GTGCCAAGTG AAAGACCATG AAGACTCATC TCTGCAGTGG
181      TCTAACCCTG CCCAGCAGAC TCTATACTTT GGGGAGAAAA GAGCCCTTCG AGATAATCGG
241      ATTCAGCTGG TTAGCTCCAC CCCGCATGAG CTCAGCATCA GCATCAGCAA CGTGGCACTG
301      GCCGACGAGG GCGAGTACAC ATGCTCCATC TTCACTATGC CTGTGCGGAC CGCCAAGTCC
361      CTCGTCACTG TGCTCGGAAT CCCACAGAAA CCCATAATCA CTGGTTATAA GTCATCGTTG
421      CGGGAAAAGG AGACAGCCAC TCTAAATTGT CAGTCTTCTG GGAGCAAACC TGCAGCCCAG
481      CTCGCCTGGA GGAAAGGTGA CCAAGAACTC CACGGGGACC AGACGCGAAT CCAGGAAGAT
541      CCCAATGGGA AAACCTTCAC TGTGAGCAGC TCGGTGTCAT TCCAGGTTAC CCGGGATGAT
601      GATGGAGCAA ACGTCGTGTG CTCTGTGAAC CATGAATCTC TGAAGGGAGC TGACAGATCC
661      ACCTCTCAGC GCATTGAAGT GTTATACACA CCAACAGCCA TGATTAGGCC AGAACCTGCT
721      CATCCTCGTG AAGGCCAGAA GCTGTTGTTA CATTGTGAGG GGCGTGGCAA TCCAGTCCCT
781      CAGCAGTACG TGTGGGTAAA AGAAGGCAGC GAGCCACCCC TCAAGATGAC CCAAGAGAGT
841      GCACTCATCT TCCCATTTTT GAACAAAAGT GACAGTGGCA CCTATGGCTG TACAGCCACG
901      AGCAACATGG GCAGCTATAC AGCCTACTTC ACTCTCAATG TCAACGACCC TAGTCCAGTG
961      CCCTCATCCT CCAGTACTTA CCACGCCATC ATTGGAGGGA TCGTGGCTTT CATCGTCTTC
1021     CTGCTGCTCA TTCTGCTCAT TTTCCTTGGA CACTATTTGA TCCGGCACAA AGGAACGTAC
1081     CTGACACACG AAGCGAAGGG CTCCGACGAC GCCCCAGATG CAGATACGGC CATCATCAAC
1141     GCAGAAGGCG GGCAGTCAGG CGGGGATGAC AAGAAGGAAT ATTTCATCTA G

 

Quality Control:cDNA sequencing results is available upon request.