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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human CA7 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG12147-G-F|
|Human CA7 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG12147-G-H|
|Human CA7 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG12147-G-M|
|Human CA7 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG12147-G-N|
|Human CA7 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG12147-G-Y|
Carbonic anhydrase 7, also known as carbonate dehydratase VII, carbonic anhydrase VII, CA-VII and CA7, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases show extensive diversity in tissue distribution and in their subcellular localization. CA7 / CA-VII is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms.