Human CA1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12145-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CA1 cDNA Clone Datasheet

CA1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC027890
cDNA Size:	786
Gene Synonym:	CAB; CA-I; Car1;CA1
Source:	Human

CA1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens carbonic anhydrase I DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CA1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CA1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAAGTC CAGACTGGGG ATATGATGAC AAAAATGGTC CTGAACAATG GAGCAAGCTG
61       TATCCCATTG CCAATGGAAA TAACCAGTCC CCTGTTGATA TTAAAACCAG TGAAACCAAA
121      CATGACACCT CTCTGAAACC TATTAGTGTC TCCTACAACC CAGCCACAGC CAAAGAAATT
181      ATCAATGTGG GGCATTCCTT CCATGTAAAT TTTGAGGACA ACGATAACCG ATCAGTGCTG
241      AAAGGTGGTC CTTTCTCTGA CAGCTACAGG CTCTTTCAGT TCCATTTTCA CTGGGGCAGT
301      ACAAATGAGC ATGGTTCAGA ACATACAGTG GATGGAGTCA AATATTCTGC CGAGCTTCAC
361      GTAGCTCACT GGAATTCTGC AAAGTACTCC AGCCTTGCTG AAGCTGCCTC AAAGGCTGAT
421      GGTTTGGCAG TTATTGGTGT TTTGATGAAG GTTGGTGAGG CCAACCCAAA GCTGCAGAAA
481      GTACTTGATG CCCTCCAAGC AATTAAAACC AAGGGCAAAC GAGCCCCATT CACAAATTTT
541      GACCCCTCTA CTCTCCTTCC TTCATCCCTG GATTTCTGGA CCTACCCTGG CTCTCTGACT
601      CATCCTCCTC TTTATGAGAG TGTAACTTGG ATCATCTGTA AGGAGAGCAT CAGTGTCAGC
661      TCAGAGCAGC TGGCACAATT CCGCAGCCTT CTATCAAATG TTGAAGGTGA TAACGCTGTC
721      CCCATGCAGC ACAACAACCG CCCAACCCAA CCTCTGAAGG GCAGAACAGT GAGAGCTTCA
781      TTTTGA

Quality Control:cDNA sequencing results is available upon request.