Human C9ORF11 Gene cDNA Clone / ORF Clone

C9ORF11 cDNA Clone Datasheet

C9ORF11 cDNA Clone Product Information

• Gene Bank Ref.ID: BC014307
• cDNA Size: 384
• Gene Synonym: AFAF; equatorin; C9orf11
• Source: Human

C9ORF11 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens chromosome 9 open reading frame 11 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

C9ORF11 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C9ORF11 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C9ORF11 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATTTTA TATTGTTTAT TTTTATACCT GGAGTTTTTT CCTTAAAAAG TAGCACTTTG
61       AAGCCTACTA TTGAAGCATT GCCTAATGTG CTACCTTTAA ATGAAGACGT TAATAAGCAG
121      GAAGAAAAGA ATGAAGATCA TACTCCCAAT TATGCTCCTG CTAATGAGAA AAATGGCAAT
181      TATTATAAAG ATATAAAACA ATATGTGTTC ACAACACAAA ATCCAAATGG CACTGAGTCT
241      GAAATATCTG TGAGAGCCAC AACTGACCTG AATTTTGCTC TAAAAAACGA TAAAACTGTC
301      AATGCAACTA CATATGAAAA ATCCACCATT GAAGAAGAAA CAACTACTAG CGAACCCTCT
361      CATAAAAATA TTCAAAGTAT TTAA

 

Quality Control:cDNA sequencing results is available upon request.