C7 / Complement component 7 Protein (His Tag)

Complement component 7 Protein Datasheet

C7 / Complement component 7 Protein Product Information

• Synonym: C7
• Protein Construction: A DNA sequence encoding the human C7 (P10643)(Met1-Gln843 )with a C-terminal polyhistidine tag was expressed.
• Source: Human
• Expression Host: Human Cells

C7 / Complement component 7 Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 23
• Molecular Mass: The recombinant human C7 comprises 832 amino acids and has a predicted molecular mass of 92.6 kDa. The apparent molecular mass of the protein is approximately 92-98 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: C7 / Complement component 7 protein

C7 / Complement component 7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

C7 / Complement component 7 Protein Description

Complement component 7 is a member of the complement C6/C7/C8/C9 family. It contains 1 EGF-like domain, 1 LDL-receptor class A domain, 1 MACPF domain, 2 Sushi (CCP/SCR) domains and 2 TSP type-1 domains. As a component of the complement system. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection. As a constituent of MAC, it plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Complement component 7 serves as a membrane anchor. Defects in C7 are a cause of complement component 7 deficiency (C7D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.

References

1. Bossi F. et al., 2009, Blood. 113 (15): 3640-8.
2. Kuijpers TW. et al., 2010, Mol Immunol. 47 (4): 671-7.
3. Thomas AD. et al., 2012, Mol Immunol. 50 (1-2): 57-9.