C6 / complement component 6 Protein (His Tag)

Complement component 6 Protein Datasheet

C6 / Complement component 6 Protein Product Information

• Synonym: C6
• Protein Construction: A DNA sequence encoding the human C6 (AAA59668.1) (Met 1- Ala 934) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

C6 / Complement component 6 Protein QC Testing

• Purity: >90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Cys 22
• Molecular Mass: The secreted recombinant human C6 comprises 924 amino acids and has a predicted molecular mass of 104 kDa. The apparent molecular mass of rh C6 is approximately 110 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: C6 / Complement component 6 protein

C6 / Complement component 6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

C6 / Complement component 6 Protein Description

Complement component 6 (C6) is a protein involved in the complement system. C6 is a glycoprotein and it has two oligosaccharide groups attached to asparagines located near the amino and the carboxyl termini of the molecule. C6 is a component of complement cascade. It is part of the membrane attack complex which can insert into the cell membrane and cause cell to lyse. As a constituent of the membrane attack complex (MAC), C6 plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. The molecular architecture of human complement component C6 was elucidated at several levels of structural organization. Defects in C6 are the cause of complement component 6 deficiency (C6D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitides.

References

1. Lengweiler S. et al., 1997, Biochim Biophys Acta. 1342 (1): 13-8.
2. Chakravarti DN. et al., 1989, Proc Natl Acad Sci. 86 (8): 2799-803.
3. DiScipio RG. et al., 1989, J Biol Chem. 264 (27): 16197-206.