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Rat C5AR1 Gene cDNA Clone (full-length ORF Clone)
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Rat C5AR1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
RG80336-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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C5AR1 cDNA Clone Datasheet

 

C5AR1 cDNA Clone Product Information

Gene Bank Ref.ID: NM_053619.1

cDNA Size:

1059

Gene Synonym:

C5r1; C5ar1

Source: Rat

C5AR1 cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus complement component 5a receptor 1 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

C5AR1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C5AR1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C5AR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACCCCA TAAGTAACGA CAGCAGTGAA ATCACCTATG ATTACTCTGA TGGAACCCCA
61       AATCCTGACA TGCCTGCAGA TGGCGTTTAC ATTCCGAAGA TGGAACCTGG GGACATTGCA
121      GCCCTTATTA TCTACTTGGC CGTGTTCCTG GTAGGAGTTA CCGGAAATGC CCTGGTGGTG
181      TGGGTGACAG CCTTTGAGGC CAAACGTACT GTCAACGCCA TTTGGTTTCT GAATCTGGCG
241      GTGGCCGACC TCCTCTCGTG CTTGGCACTG CCTATCCTGT TTACGTCCAT TGTAAAGCAT
301      AACCACTGGC CCTTCGGTGA CCAGGCCTGT ATAGTCCTGC CCTCGCTCAT TCTGCTCAAC
361      ATGTACTCCA GCATCCTGCT GCTGGCCACT ATTAGTGCCG ACCGTTTCCT GCTGGTGTTC
421      AAACCCATCT GGTGTCAGAA GTTCCGCCGG CCTGGCCTGG CCTGGATGGC TTGCGGAGTA
481      ACCTGGGTCT TAGCATTGCT CCTCACCATT CCGTCCTTCG TGTTCCGGCG GATACATAAG
541      GACCCCTACT CAGATAGCAT TCTATGTAAT ATTGACTATA GTAAGGGTCC GTTCTTCATA
601      GAGAAGGCTA TAGCCATCCT GCGGCTGATG GTGGGTTTCG TGTTGCCTCT GCTCACTCTT
661      AACATCTGCT ACACCTTCCT CCTGATCCGG ACCTGGAGTC GCAAGGCCAC GCGCTCCACC
721      AAGACGCTCA AAGTGGTGAT GGCGGTGGTC ACATGTTTCT TTGTCTTCTG GCTACCCTAC
781      CAGGTAACAG GGGTGATATT AGCTTGGCTG CCCCGGTCCT CATCCACTTT TCAGTCAGTG
841      GAGAGGCTGA ACTCCTTGTG CGTTTCCCTG GCCTACATCA ACTGCTGTGT TAACCCTATC
901      ATCTACGTCA TGGCTGGCCA GGGTTTCCAT GGGAGACTCC GAAGGTCACT CCCCAGCATC
961      ATACGAAACG TGCTCTCTGA GGACTCCTTG GGCAGGGACA GCAAGAGTTT CACTCGGTCC
1021     ACGATGGATA CCTCCACCCA GAAGAGTCAG GCAGTGTAG

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"