Human C5AR1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12154-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

C5AR1 cDNA Clone Datasheet

C5AR1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC008982
cDNA Size:	1053
Gene Synonym:	C5A;C5AR;C5R1;CD88;C5AR1
Source:	Human

C5AR1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens complement component 5a receptor 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

C5AR1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C5AR1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C5AR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTCCT TCAATTATAC CACCCCTGAT TATGGGCACT ATGATGACAA GGATACCCTG
61       GACCTCAACA CCCCTGTGGA TAAAACTTCT AACACGCTGC GTGTTCCAGA CATCCTGGCC
121      TTGGTCATCT TTGCAGTCGT CTTCCTGGTG GGAGTGCTGG GCAATGCCCT GGTGGTCTGG
181      GTGACGGCAT TCGAGGCCAA GCGGACCATC AATGCCATCT GGTTCCTCAA CTTGGCGGTA
241      GCCGACTTCC TCTCCTGCCT GGCGCTGCCC ATCTTGTTCA CGTCCATTGT ACAGCATCAC
301      CACTGGCCCT TTGGCGGGGC CGCCTGCAGC ATCCTGCCCT CCCTCATCCT GCTCAACATG
361      TACGCCAGCA TCCTGCTCCT GGCCACCATC AGCGCCGACC GCTTTCTGCT GGTGTTTAAA
421      CCCATCTGGT GCCAGAACTT CCGAGGGGCC GGCTTGGCCT GGATCGCCTG TGCCGTGGCT
481      TGGGGTTTAG CCCTGCTGCT GACCATACCC TCCTTCCTGT ACCGGGTGGT CCGGGAGGAG
541      TACTTTCCAC CAAAGGTGTT GTGTGGCGTG GACTACAGCC ACGACAAACG GCGGGAGCGA
601      GCCGTGGCCA TCGTCCGGCT GGTCCTGGGC TTCCTGTGGC CTCTACTCAC GCTCACGATT
661      TGTTACACTT TCATCCTGCT CCGGACGTGG AGCCGCAGGG CCACGCGGTC CACCAAGACA
721      CTCAAGGTGG TGGTGGCAGT GGTGGCCAGT TTCTTTATCT TCTGGTTGCC CTACCAGGTG
781      ACGGGGATAA TGATGTCCTT CCTGGAGCCA TCGTCACCCA CCTTCCTGCT GCTGAATAAG
841      CTGGACTCCC TGTGTGTCTC CTTTGCCTAC ATCAACTGCT GCATCAACCC CATCATCTAC
901      GTGGTGGCCG GCCAGGGCTT CCAGGGCCGA CTGCGGAAAT CCCTCCCCAG CCTCCTCCGG
961      AACGTGTTGA CTGAAGAGTC CGTGGTTAGG GAGAGCAAGT CATTCACGCG CTCCACAGTG
1021     GACACTATGG CCCAGAAGAC CCAGGCAGTG TAG

Quality Control:cDNA sequencing results is available upon request.