Mouse C1QB Gene cDNA Clone / ORF Clone

C1QB cDNA Clone Datasheet

C1QB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009777.2
• cDNA Size: 762
• Gene Synonym: C1QB
• Source: Mouse

C1QB cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus complement component 1, q subcomponent, beta polypeptide DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for five point mutations: 343 G/A resulting in the amino acid Gly substitution by Arg, 48 T/C, 132 T/C, 549 T/G and 618 G/A not causing the amino acid variation.

C1QB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C1QB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C1QB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGACAC AGTGGGGTGA GGTCTGGACA CACCTGTTAC TGCTGCTCCT AGGTTTTCTC
61       CATGTGTCCT GGGCCCAAAG CAGCTGCACC GGGCCCCCTG GCATCCCTGG CATCCCTGGG
121      GTCCCTGGGG TCCCTGGCTC TGATGGCCAA CCAGGCACTC CAGGGATAAA GGGGGAGAAA
181      GGGCTCCCTG GACTGGCTGG AGACCTTGGT GAGTTTGGAG AGAAAGGGGA CCCAGGGATC
241      CCTGGGACTC CAGGCAAAGT TGGCCCTAAG GGTCCCGTCG GCCCTAAGGG TACTCCAGGC
301      CCCTCTGGAC CCCGCGGTCC CAAAGGCGAT TCTGGGGACT ACAGGGCTAC ACAGAAAGTC
361      GCCTTCTCTG CCCTGAGGAC CATCAACAGC CCCTTGCGAC CGAACCAGGT CATTCGCTTC
421      GAAAAGGTGA TCACCAACGC GAACGAGAAC TATGAGCCAC GCAACGGCAA GTTCACCTGC
481      AAGGTGCCTG GCCTCTACTA CTTCACCTAT CATGCCAGCT CCCGGGGCAA CCTGTGTGTG
541      AATCTCGTGC GTGGCCGCGA TCGGGACAGC ATGCAGAAAG TAGTCACCTT CTGTGACTAT
601      GCCCAGAACA CCTTCCAAGT GACCACAGGT GGGGTAGTCT TGAAGCTAGA GCAAGAGGAG
661      GTTGTTCACC TGCAGGCCAC AGACAAGAAC TCCCTCCTGG GCATTGAGGG TGCCAACAGC
721      ATCTTCACTG GCTTTCTGCT TTTCCCTGAC ATGGATGCGT AA

 

Quality Control:cDNA sequencing results is available upon request.