Mouse HABP1 / C1QBP Protein (His Tag)

Mouse Complement Component 1 Q Subcomponent-binding Protein

Mouse C1QBP Protein Product Information

• Synonym: SDPR
• Protein Construction: A DNA sequence encoding the mature form of mouse C1QBP ( NP_031599.2 ) ( Leu 72 - Gln 279 ) was expressed, with a polyhistide tag at the N-terminus
• Source: Mouse
• Expression Host: E. coli

Mouse C1QBP Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse C1QBP consisting of 215 amino acids and has a calculated molecular mass of 24.7 kDa. rmC1QBP migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: C1QBP protein

Mouse C1QBP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Mouse C1QBP Protein Description

Mouse complement component 1 Q subcomponent-binding protein, mitochondrial, also known as GC1q-R protein, Glycoprotein gC1qBP, Hyaluronan-binding protein 1, Mitochondrial matrix protein p32, p33 and C1QBP, is a nucleus protein which belongs to the MAM33 family. Mouse C1QBP ( p33, p32, GC1q-R, TAP ) is a ubiquitously expressed, multiligand-binding, multicompartmental cellular protein involved in various ligand-mediated cellular responses. C1QBP is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein. Although expressed on the surface of cells, an intriguing feature of the membrane-associated form of C1QBP is that its translated amino acid sequence does not predict the presence of either a sequence motif compatible with a transmembrane segment or a consensus site for a glycosyl-phosphatidylinositol anchor. Moreover, the N-terminal sequence of the pre-pro-protein C1QBP contains a motif that targets the molecule to the mitochondria and as such was deemed unlikely to be expressed on the surface. C1QBP also interacts with HIV-1 Tat.

References

1. Jiang J., et al., 1999, Proc. Natl. Acad. Sci. USA. 96: 3572-7.
2. Beatch M.D., et al., 2000, J. Virol. 74: 5569-76.
3. Ghebrehiwet,B. et al., 2001,Immunol Rev.180 :65-77.
4. Lehner, Ben, et al.,2004, Genomics. 83 (1): 153-67.
5. Berro R., et al., 2006, J. Virol. 80: 3189-204.