Anti-C1QBP Antibody

Cat: 50199-RP02
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All C1QBP Reagents
CRO Services
Product recommended

All C1QBP Reagents
Anti-C1QBP Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-C1QBP Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse C1QBP
Immunogen
Recombinant Mouse C1QBP / HABP1 protein (Catalog#50199-M07E)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse C1QBP / HABP1 (rM C1QBP / HABP1; Catalog#50199-M07E; NP_031599.2; Leu 72-Gln 279). C1QBP / HABP1 specific IgG was purified by Mouse C1QBP / HABP1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-C1QBP Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-C1QBP Antibody (Rabbit Polyclonal antibody) Images
Mouse C1QBP/HABP1 Immunoprecipitation(IP) 8224

Mouse C1QBP was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-Mouse C1QBP rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse C1QBP rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 35 kDa

Mouse C1QBP/HABP1 Western blot (WB) 13654

Anti-C1QBP rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:35 kDa

Anti-C1QBP Antibody Alternative Names
Anti-AA407365 Antibody;Anti-AA986492 Antibody;Anti-D11Wsu182e Antibody;Anti-gC1qBP Antibody;Anti-HABP1 Antibody;Anti-P32 Antibody
C1QBP Background Information

Hyaluronan binding protein 1 (HABP1), also known as p32 or gC1qR, is a ubiquitously expressed multifunctional phospho-protein implicated in cell signalling. Hyaluronan-binding protein 1 (HABP1) /p32/gC1qR was characterized as a highly acidic and oligomeric protein, which binds to different ligands like hyaluronan, C1q, and mannosylated albumin. The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro. HABP1 overexpressing cells showed extensive vacuolation and reduced growth rate, which was corrected by frequent medium replenishment. Further investigation revealed that HABP1 overexpressing cells undergo apoptosis, and they failed to enter into the S-phase. The sperm surface HABP1 level can be correlated with the degree of sperm motility.Hyaluronan binding protein 1 (HABP1) was reported to be present on human sperm surface and its involvement in fertilization has already been elucidated: decreased HABP1 level may be associated with low motility of sperms, which in turn might cause infertility in the patient. HABP1 also is an endogenous substrate for MAP kinase and upon mitogenic stimulation it is translocated to the nucleus in a MAP kinase-dependent manner.

Full Name
complement component 1, q subcomponent binding protein
References
  • Meenakshi J, et al. (2003) Constitutive expression of hyaluronan binding protein 1 (HABP1/p32/gC1qR) in normal fibroblast cells perturbs its growth characteristics and induces apoptosis. Biochemicaland Biophysical Research Communication. 300(3): 686-93.
  • Majumdar M, et al. (2002) Hyaluronan Binding Protein 1 (HABP1) /C1QBP/p32 Is an Endogenous Substrate for MAP Kinase and Is Translocated to the Nucleus upon Mitogenic Stimulation. Biochemical and Biophysical Research Communications. 291(4): 829-37.
  • Ghosh I, et al. (2002) Reduction in the level of hyaluronan binding protein 1 (HABP1) is associated with loss of sperm motility. Journal of Reproductive Immunology. 53(1-2): 45-54.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-C1QBP Antibody Rabbit Polyclonal ELISA 50199-RP01
    Anti-C1QBP Antibody Rabbit Monoclonal ELISA 50199-R001
    Anti-C1QBP Antibody Rabbit Monoclonal WB,IP 50199-R106
    Anti-C1QBP Antibody Rabbit Polyclonal WB,ELISA,IHC-P,ICC/IF,IF,IP 50199-T62
    Anti-C1QBP Antibody Mouse Monoclonal FCM,ICC/IF,IF 11874-MM09
    Anti-C1QBP Antibody Mouse Monoclonal WB,ELISA,ICC/IF,IF,IP 11874-MM04
    Anti-C1QBP Antibody Rabbit Polyclonal WB,IP 11874-T36
    Anti-C1QBP Antibody Rabbit Polyclonal ELISA 11874-RP01
    info info
    添加购物车成功! 添加购物车失败!