Human C12ORF49 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13350-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

C12ORF49 cDNA Clone Datasheet

C12ORF49 cDNA Clone Product Information

Gene Bank Ref.ID:	BC019843
cDNA Size:	618
Gene Synonym:	C12orf49
Source:	Human

C12ORF49 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens chromosome 12 open reading frame 49 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

C12ORF49 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C12ORF49 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C12ORF49 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTGAACC TGGCGGCCAT GGTGTGGCGC CGGCTTCTGC GGAAGAGGTG GGTGCTCGCC
61       CTGGTCTTCG GGCTGTCGCT CGTCTACTTC CTCAGCAGCA CCTTCAAGCA GGAGGAGAGG
121      GCAGTGAGAG ATAGGAATCT CCTCCAGGTT CATGACCATA ATCAGCCCAT CCCGTGGAAA
181      GTGCAGTTTA ACTTGGGCAA TAGCAGTCGT CCGAGCAATC AGTGCCGCAA CTCCATTCAA
241      GGGAAGCACC TCATCACGGA TGAACTCGGC TACGTTTGCG AGAGGAAGGA TTTGCTGGTA
301      AATGGCTGCT GTAATGTCAA CGTCCCTAGC ACGAAGCAGT ACTGCTGTGA TGGCTGCTGG
361      CCCAACGGCT GCTGCAGCGC CTATGAGTAC TGTGTCTCCT GCTGCCTGCA GCCCAACAAG
421      CAACTTCTCC TGGAGCGCTT CCTCAACCGG GCAGCCGTGG CATTCCAGAA CCTCTTCATG
481      GCAGTCGAAG ATCACTTTGA GTTGTGCCTG GCCAAATGCA GGACCTCATC TCAGAGCGTG
541      CAGCATGAGA ACACCTACCG GGACCCCATA GCAAAGTATT GCTATGGAGA AAGCCCGCCC
601      GAGCTCTTCC CCGCTTGA

Quality Control:cDNA sequencing results is available upon request.