GI24 / B7H5 Protein (His Tag)

Chromosome 10 open reading frame 54 Protein Datasheet

C10orf54 / GI24 Protein Product Information

• Synonym: Gi24, VISTA, B7H5, C10orf54
• Protein Construction: A DNA sequence encoding the human Gi24 (AAH20568.1) (Met1-Ala194) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

C10orf54 / GI24 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 33
• Molecular Mass: The recombinant human Gi24 consists of 173 amino acids and predicts a molecular mass of 19.6 KDa. It migrates as an approximately 38-42 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: C10orf54 / GI24 protein

C10orf54 / GI24 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

C10orf54 / GI24 Protein Description

C10orf54, also known as GI24, is a member of the immunoglobulin superfamily. As a transmembrane molecule, C10orf54 is expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, C10orf54 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

References

1. Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
2. Deloukas P. et al., 2004, Nature. 429 (6990): 375-81.
3. Colland F. et al., 2004, Genome Res. 14 (7): 1324-32.