Human C10ORF54 Gene cDNA Clone / ORF Clone

C10ORF54 cDNA Clone Datasheet

C10ORF54 cDNA Clone Product Information

• Gene Bank Ref.ID: BC020568
• cDNA Size: 936
• Gene Synonym: GI24; B7-H5; SISP1; PP2135; C10orf54
• Source: Human

C10ORF54 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens chromosome 10 open reading frame 54 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

C10ORF54 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C10ORF54 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C10ORF54 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCGTCC CCACGGCCCT GGAGGCCGGC AGCTGGCGCT GGGGATCCCT GCTCTTCGCT
61       CTCTTCCTGG CTGCGTCCCT AGGTCCGGTG GCAGCCTTCA AGGTCGCCAC GCCGTATTCC
121      CTGTATGTCT GTCCCGAGGG GCAGAACGTC ACCCTCACCT GCAGGCTCTT GGGCCCTGTG
181      GACAAAGGGC ACGATGTGAC CTTCTACAAG ACGTGGTACC GCAGCTCGAG GGGCGAGGTG
241      CAGACCTGCT CAGAGCGCCG GCCCATCCGC AACCTCACGT TCCAGGACCT TCACCTGCAC
301      CATGGAGGCC ACCAGGCTGC CAACACCAGC CACGACCTGG CTCAGCGCCA CGGGCTGGAG
361      TCGGCCTCCG ACCACCATGG CAACTTCTCC ATCACCATGC GCAACCTGAC CCTGCTGGAT
421      AGCGGCCTCT ACTGCTGCCT GGTGGTGGAG ATCAGGCACC ACCACTCGGA GCACAGGGTC
481      CATGGTGCCA TGGAGCTGCA GGTGCAGACA GGCAAAGATG CACCATCCAA CTGTGTGGTG
541      TACCCATCCT CCTCCCAGGA GAGTGAAAAC ATCACGGCTG CAGCCCTGGC TACGGGTGCC
601      TGCATCGTAG GAATCCTCTG CCTCCCCCTC ATCCTGCTCC TGGTCTACAA GCAAAGGCAG
661      GCAGCCTCCA ACCGCCGTGC CCAGGAGCTG GTGCGGATGG ACAGCAACAT TCAAGGGATT
721      GAAAACCCCG GCTTTGAAGC CTCACCACCT GCCCAGGGGA TACCCGAGGC CAAAGTCAGG
781      CACCCCCTGT CCTATGTGGC CCAGCGGCAG CCTTCTGAGT CTGGGCGGCA TCTGCTTTCG
841      GAGCCCAGCA CCCCCCTGTC TCCTCCAGGC CCCGGAGACG TCTTCTTCCC ATCCCTGGAC
901      CCTGTCCCTG ACTCTCCAAA CTTTGAGGTC ATCTAG

 

Quality Control:cDNA sequencing results is available upon request.