Anti-C1 inhibitor Antibody

Cat: 10995-R018
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Anti-C1 inhibitor Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-C1 inhibitor Antibody
Validated applications
WB,ELISA,ELISA(Det),IP
Species reactivity
Reacts with: Human
Specificity
Human C1 inhibitor
No cross-reactivity in ELISA with Human SerpinA1 / A1AT Human SerpinA3 / SERPINA3 / AACT Human SerpinA5 / PROCI Human SerpinC1 /SERPINC1/ Antithrombin-III Human SerpinD1 / SERPIND1 / HC2 Human SerpinF1 / SERPINF1 / PEDF Human SerpinF2 / SERPINF2 Human SerpinI1 / SERPINI1 / Protease inhibitor 12
Immunogen
Recombinant Human SerpinG1 protein (Catalog#10995-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SerpinG1 (rh SerpinG1; Catalog#10995-H08H; NP_000053.2; Met 1-Ala 500).
Source
Monoclonal Rabbit IgG Clone #18
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-C1 inhibitor Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 0.5-2 μL/mg of lysate  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human C1 inhibitor / SerpinG1 in ELISA pair set (Catalog: # SEK10995). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10995-MM06).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-C1 inhibitor Antibody (Rabbit Monoclonal antibody) Images
Human C1 inhibitor/SerpinG1 Immunoprecipitation(IP) 6177

SERPING1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SERPING1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SERPING1 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size: 100 kDa

Human C1 inhibitor/SerpinG1 Western blot (WB) 13318

Anti-SerpinG1 rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:71 kDa

Anti-C1 inhibitor Antibody Alternative Names
Anti-C1IN Antibody;Anti-C1INH Antibody;Anti-C1NH Antibody;Anti-HAE1 Antibody;Anti-HAE2 Antibody
C1 inhibitor Background Information

Plasma protease C1 inhibitor, also known as C1-inhibiting factor, C1-INH, C1 esterase inhibitor, SERPING1 and C1IN, is a serine proteinase inhibitor (serpin) that regulates activation of both the complement and contact systems. By its C-terminal part (serpin domain), characterized by three beta-sheets and an exposed mobile reactive loop, C1-INH binds, and blocks the activity of its target proteases. The N-terminal end (nonserpin domain) confers to C1-INH the capacity to bind lipopolysaccharides and E-selectin. Owing to this moiety, C1-INH intervenes in regulation of the inflammatory reaction. The heterozygous deficiency of C1-INH results in hereditary angioedema (HAE). Owing to its ability to modulate the contact and complement systems and the convincing safety profile, plasma-derived C1 inhibitor is an attractive therapeutic protein to treat inflammatory diseases other than HAE. Deficiency of C1 inhibitor results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa. C1 inhibitor may prove useful in a variety of other diseases including septic shock, reperfusion injury, hyperacute transplant rejection, traumatic and hemorrhagic shock, and the increased vascular permeability associated with thermal injury, interleukin-2 therapy and cardiopulmonary bypass.

Full Name
serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
References
  • Davis AE 3rd. et al. (2004) Biological effects of C1 inhibitor. Drug News Perspect. 17(7): 439-46.
  • Cicardi M, et al. (2005) C1 inhibitor: molecular and clinical aspects. Springer Semin Immunopathol. 27(3): 286-98.
  • Wouters D, et al. (2008) C1 inhibitor: just a serine protease inhibitor? New and old considerations on therapeutic applications of C1 inhibitor. Expert Opin Biol Ther. 8(8): 1225-40.
  • Cugno M, et al. (2009) C1-inhibitor deficiency and angioedema: molecular mechanisms and clinical progress. Trends Mol Med. 15(2): 69-78.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-C1 inhibitor Antibody Mouse Monoclonal ELISA 10995-MM03
    Anti-C1 inhibitor Antibody Rabbit Polyclonal ELISA 10995-RP01
    Anti-C1 inhibitor Antibody Rabbit Polyclonal WB,ELISA,IHC-P 10995-RP02
    Anti-C1 inhibitor Antibody Mouse Monoclonal ELISA(Cap) 10995-MM06
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