SerpinG1 / C1IN Antibody

Plasma Protease C1 Inhibitor / C1-inhibiting Factor Antibody

SerpinG1 / C1IN Antibody Product Information

• Immunogen: Recombinant human SerpinG1 protein ( Catalog#10995-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 18
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human SerpinG1 ( rh SerpinG1 ; Catalog#10995-H08H ; NP_000053.2 ; Met 1 - Ala 500 ).

SerpinG1 / C1IN Antibody Usage Guide

• Specificity: Human SerpinG1 / C1IN
• No cross-reactivity in ELISA with

  Human SerpinA1 / A1AT

  Human SerpinA3 / SERPINA3 / AACT

  Human SerpinA5 / PROCI

  Human SerpinC1 / SERPINC1 / Antithrombin-III

  Human SerpinD1 / SERPIND1 / HC2

  Human SerpinF1 / SERPINF1 / PEDF

  Human SerpinF2 / SERPINF2

  Human SerpinI1 / SERPINI1 / Protease inhibitor 12

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human SERPING1 in WB. Using a DAB detection system, the detection limit for human SERPING1 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human SERPING1. The detection limit for human SERPING1 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinG1 / C1IN Antibody Background

Plasma protease C1 inhibitor, also known as C1-inhibiting factor, C1-INH, C1 esterase inhibitor, SERPING1 and C1IN, is a serine protease inhibitor protein. C1-INH is the largest member among the serpin class of proteins. Remarkably, C1-INH has a 2-domain structure, unlike most family members. The C-terminal serpin domain is similar to other serpins, and this part of C1-INH provides the inhibitory activity. The N-terminal domain is not essential for C1-INH to inhibit proteinases and has no similarity to other proteins. The main function of C1-INH is the inhibition of the complement system to prevent spontaneous activation. C1-INH is an acute phase protein and circulates in blood at levels of around 0.25 g/L, whose levels rise 2-fold during inflammation. C1-INH irreversibly binds to and inactivates C1r and C1s proteinases in the C1 complex of classical pathway of complement. C1-INH prevents the proteolytic cleavage of later complement components C4 and C2 by C1 and MBL. Although named after its complement inhibitory activity, C1-INH also inhibits proteinases of the fibrinolytic, clotting, and kinin pathways. Most notably, C1-INH is the most important physiological inhibitor of plasma kallikrein, fXIa and fXIIa.

References

1. Theriault, A. et al., 1990, Hum. Genet. 84 (5): 477-9. 
2. Davis, AE. et al., 2004, Drug News Perspect. 17 (7): 439-46. 
3. Koles, K. et al., 2004, Glycobiology. 14 (1): 51-64. 
4. Cicardi, M. et al., 2005, Springer Semin. Immunopathol. 27(3): 286-98. 
5. Bernstein, JA. et al., 2008, Ann. Allergy Asthma Immunol.100: S41-6.