Mouse CSK Gene cDNA Clone / ORF Clone

CSK cDNA Clone Datasheet

CSK cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007783.2
• cDNA Size: 1353
• Gene Synonym: AW212630; Csk
• Source: Mouse

CSK cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus c-src tyrosine kinase DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 642 C/T not causing the amino acid variation.

CSK cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CSK cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CSK cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGGCAA TACAGGCCGC CTGGCCATCC GGTACAGAAT GTATTGCCAA GTACAACTTC
61       CATGGCACTG CTGAGCAAGA CCTTCCCTTC TGCAAAGGAG ATGTGCTCAC CATCGTGGCT
121      GTCACCAAGG ACCCCAACTG GTACAAAGCC AAAAACAAAG TGGGCCGTGA GGGCATCATC
181      CCAGCCAACT ATGTCCAGAA GCGTGAGGGT GTGAAGGCAG GCACCAAACT CAGCCTTATG
241      CCCTGGTTCC ACGGCAAGAT CACACGGGAG CAGGCCGAGC GGCTTCTTTA CCCACCAGAG
301      ACAGGCCTGT TCCTCGTGCG GGAAAGCACC AACTACCCTG GAGACTACAC ACTGTGTGTG
361      AGCTGTGAGG GCAAGGTGGA GCACTACCGC ATCATGTATC ATGCGAGCAA GCTGAGCATT
421      GATGAGGAGG TGTACTTTGA GAACCTCATG CAGCTGGTGG AGCACTACAC CACAGATGCC
481      GATGGACTCT GCACTCGCCT CATCAAACCA AAGGTCATGG AGGGCACCGT GGCGGCCCAG
541      GATGAGTTCT ACCGCAGTGG CTGGGCACTG AACATGAAGG AACTGAAGCT GCTACAGACA
601      ATAGGGAAGG GGGAGTTTGG AGATGTGATG CTGGGGGATT ATCGGGGCAA CAAAGTTGCA
661      GTCAAGTGCA TCAAGAATGA CGCAACTGCC CAGGCCTTCC TGGCTGAAGC CTCCGTCATG
721      ACGCAACTTC GGCACAGCAA CCTCGTCCAG CTGCTGGGTG TGATTGTGGA GGAGAAGGGT
781      GGGCTCTACA TCGTCACAGA GTACATGGCC AAGGGGAGTT TGGTGGACTA TCTTCGATCA
841      CGTGGTCGTT CGGTGCTAGG TGGAGACTGT CTCCTCAAAT TCTCATTAGA CGTCTGTGAA
901      GCCATGGAGT ACCTGGAGGG TAACAATTTT GTGCACCGGG ACTTGGCTGC CCGGAATGTG
961      CTGGTGTCTG AAGACAACGT GGCCAAAGTC AGTGACTTTG GCCTCACTAA GGAAGCCTCC
1021     AGCACTCAGG ACACAGGCAA GCTGCCAGTC AAATGGACAG CGCCTGAAGC CTTGAGAGAG
1081     AAGAAATTTT CCACCAAGTC TGATGTGTGG AGTTTCGGAA TCCTTCTCTG GGAAATCTAT
1141     TCCTTCGGGC GAGTGCCTTA CCCAAGAATT CCCCTGAAGG ACGTCGTCCC TCGGGTGGAA
1201     AAGGGCTATA AGATGGACGC TCCGGATGGC TGCCCGCCCG CAGTCTACGA GGTGATGAAG
1261     AACTGCTGGC ACCTGGATGC TGCCACACGG CCCACGTTTT TGCAGCTTCG GGAACAGCTC
1321     GAGCACATCA AGACCCATGA GCTGCACCTG TGA

 

Quality Control:cDNA sequencing results is available upon request.