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Beta-Tubulin Loading Control Antibody, Mouse MAb

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Beta-TubulinAntibody Product Information
Antigen:A synthetic peptide corresponding to the N-terminus of the human beta-tubulin
Clone ID:05
Ig Type:Mouse IgG2b
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Beta-TubulinAntibody Usage Guide
Specificity:Human Beta-Tubulin
Application:WB, ICC/IF, IF

WB: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Beta-Tubulin in WB.

ICC/IF: 5-20 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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Beta-Tubulin Loading Control Antibody, Mouse MAb, Immunofluorescence

Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody (10 µg/ml) at 4℃ overnigh

Beta-Tubulin Loading Control Antibody, Mouse MAb, Western blot

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"