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>Beta-Tubulin Loading Control Antibody, Mouse MAb
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Beta-Tubulin Antibody Datasheet
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Beta-Tubulin Antibody Product Information
|Antibody Type :||Mouse Monoclonal Antibody ( Mouse mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Beta-Tubulin Antibody Usage Guide
|Western blot :||
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Beta-Tubulin in WB.
ICC/IF: 5-20 μg/mL
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Beta-Tubulin Antibody Related Products & Topics
Beta-Tubulin Antibody Background
Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart.
Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.
- Williams RC Jr. et al., 1999. Anal Biochem. 275(2): 265-7.
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- Dutcher SK. 2001. Curr Opin Cell Biol. 13(1): 49-54.