Beta-Tubulin Loading Control Antibody, Mouse MAb

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Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
Beta-Tubulin Loading Control Antibody, Mouse MAb
Validated applications
WB,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human Beta-Tubulin
Immunogen
A synthetic peptide corresponding to the N-terminus of the human beta-tubulin
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1/1000-1/2000
ICC-IF 1/50-1/200
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal antibody) Images

Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.

Beta-Tubulin Background Information

Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.
References
  • Williams RC Jr. et al., 1999. Anal Biochem. 275(2): 265-7.
  • Nogales E. et al., 1998. Nat Struct Biol. 5(6): 451-8.
  • Dutcher SK. 2001. Curr Opin Cell Biol. 13(1): 49-54.
  • Amyloid precursor-like protein 2 C-terminal fragments upregulate S100A9 gene and protein expression in BV2 cells
    Author
    Li, G;Chen, H;Cheng, L;Zhao, R;Zhao, J;Xu, Y;
    Year
    2014
    Journal
    Neural Regen Res
    Application
    WB
  • Design, synthesis and biological evaluation of shikonin thio-glycoside derivatives: new anti-tubulin agents
    Author
    HY Lin, HW Han, LF Bai, HY Qiu, DZ Yin, JL Qi…
    Year
    2014
    Journal
    RSC Advances
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