Anti-beta-Actin Antibody

Cat: 100162-RP02
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All beta-Actin Reagents
Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-beta-Actin Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human beta-Actin
Immunogen
A synthetic peptide corresponding to the C-terminus of the human beta actin.
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human beta actin, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS, pH7.4
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 0.5-5 μg/ml  
IHC-P 0.5-5 μg/mL  
IP 1-4 uL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Images
Human Beta-Actin Immunohistochemistry(IHC) 3406

Anti-ADK mouse monoclonal antibody (Cat: ab86607) at 1 µg/ml

Lane A: RAW264.7 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: NIH/3T3 Whole Cell Lysate

Lysates/proteins at 30 µg per lane.

Secondary

Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 74 kDa

Human Beta-Actin Immunohistochemistry(IHC) 3407
Immunochemical staining of human β-Actin in human hepatoma with rabbit polyclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).
Human Beta-Actin Western blot (WB) 6434
Human Beta-Actin Immunoprecipitation(IP) 14052
Anti-beta-Actin Antibody Alternative Names
Anti-BRWS1 Antibody;Anti-PS1TP5BP1 Antibody
beta-Actin Background Information
Full Name
actin, beta
Product Description Host Clonality Application Catalog# (PDF)
Anti-beta-Actin Antibody Mouse Monoclonal WB,IP 100166-MM10
Anti-beta-Actin Antibody Rabbit Polyclonal WB,IP 100166-T36
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