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Beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified

  • Beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified, Western Blot
  • Beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
  • Beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
  • Beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified,Immunoprecipitation
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Human Beta-Actin Antibody Product Information
Immunogen:A synthetic peptide corresponding to the C-terminus of the human beta actin.
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS, pH7.4
Preparation:Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human beta actin, and purified by antigen affinity chromatography.
Human Beta-Actin Antibody Usage Guide
Human Beta-Actin Antibody WB Application Image
Human Beta-Actin Antibody IHC Application Image
Beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human β-Actin in human hepatoma with rabbit polyclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).
Beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:

Anti-ADK mouse monoclonal antibody (Cat: ab86607) at 1 µg/ml

Lane A: RAW264.7 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: NIH/3T3 Whole Cell Lysate

Lysates/proteins at 30 µg per lane.

Secondary

Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 74 kDa


Other Beta-Actin Antibody Products
Beta-Actin Background

Beta-actin is an isform of actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. There are six different but highly conserved actin isoforms in vertebrates. Four of these isoforms are expressed primarily in striated and smooth muscle cells, whereas the two cytoplasmic β-actin and γ-actin isoforms are ubiquitously expressed. Conserved from birds to mammals, β-actin and γ-actin differ at only four biochemically similar amino acid residues. These amino acid differences confer unique biochemical properties between the two isoforms. Beta-actin is a major constituent of the contractile apparatus. Beta-actin, also known as a “housekeeping” protein, is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Loading controls are essential for proper interpretation of western blots. They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.

Human Beta-Actin References
  • Hanukoglu I., et al. 1983, J Mol Biol. 163(4): 673-8.
  • Mao B., et al. 2001, Nature. 411(6835): 321-5.
  • Holleran EA., et al. 2001, J Biol Chem. 276(39): 36598-605.
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