Anti-Bcl-XL Antibody (FITC)

Cat: 10455-MM07-F
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Anti-Bcl-XL Antibody (FITC) (Mouse Monoclonal antibody) General Information
Product name
Anti-Bcl-XL Antibody (FITC)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human Bcl-XL
No cross-reactivity in ELISA with E.coli cell lysate
Immunogen
Recombinant Human Bcl-XL / BCL2L1 protein (Catalog#10455-H08E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Bcl-XL / BCL2L1 (rh Bcl-XL / BCL2L1; Catalog#10455-H08E; NP_612815.1; Met 1-Arg 212) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG1 Clone #07
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-Bcl-XL Antibody (FITC) (Mouse Monoclonal antibody) Validated Applications

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Bcl-XL Antibody (FITC) (Mouse Monoclonal antibody) Images
Human BCL2L1/Bcl-XL Flow Cytometry (FC) 4914
Flow cytometric analysis of FITC anti-Human BCL2L1 (Bcl-xL) on HeLa cells. HeLa cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with FITC Mouse anti-BCL2L1 (Bcl-xL).
Anti-Bcl-XL Antibody (FITC) Alternative Names
Anti-Bcl-X Antibody;Anti-bcl-xL Antibody;Anti-BCL-XL/S Antibody;Anti-bcl-xS Antibody;Anti-BCL2L Antibody;Anti-BCLX Antibody;Anti-BCLXL Antibody;Anti-BCLXS Antibody;Anti-PPP1R52 Antibody
Bcl-XL Background Information

B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Full Name
BCL2-like 1
References
  • Vander Heiden MG, et al. (1997) Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria. Cell. 91 (5): 627-37.
  • Muchmore SW, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 381: 335-341.
  • SharoffEH, et al. (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal. 9 (9) :1405-9.
  • Zhou F, et al. (2011) Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J. 278 (3): 403-13.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Bcl-XL Antibody Rabbit Monoclonal ELISA 50012-R042
    Anti-Bcl-XL Antibody Rabbit Polyclonal ELISA 50012-RP01
    Anti-Bcl-XL Antibody Rabbit Polyclonal ELISA,IHC-P 50012-RP02
    Anti-Bcl-XL Antibody Rabbit Monoclonal WB,IP 50012-R036
    Anti-Bcl-XL Antibody Mouse Monoclonal ELISA,FCM 10455-MM07
    Anti-Bcl-XL Antibody Rabbit Monoclonal WB,ELISA,IP 10455-R016
    Anti-Bcl-XL Antibody Rabbit Polyclonal ELISA 10455-T16
    Anti-Bcl-XL Antibody Rabbit Polyclonal WB 101579-T32
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