Mouse BUB1 Gene cDNA Clone / ORF Clone

BUB1 cDNA Clone Datasheet

BUB1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009772.2
• cDNA Size: 3177
• Gene Synonym: Bub1a; C80208; D2Xrf87; AL022991; Bub1
• Source: Mouse

BUB1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 560 G/A and 621 G/A resulting in the amino acid Arg substitution by Lys,Met substitution by Ile and 240 T/C,2154 C/T,2502 T/C not causing the amino acid variation.

BUB1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BUB1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BUB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACAACC TAGAAAATGT CTTTCGCATG TTTGAAGCCC ATATGCAAAG CTACACGGGT
61       AATGACCCAC TTGGAGAATG GGAAAGCTTT ATAAAGTGGG TAGAAGAGAA TTTTCCTGAC
121      AATAAAGAAT ACTTGATGAC ATTATTAGAA CATTTAATGA AGGAATTTTT ACATAAGAAG
181      AACTACCACA ATGATTCAAG ATTCATCAAT TATTGCTTAA AATTTGCTGA GTACAACAGC
241      GACCGTCATC AGTTTTTTGA GTTTCTGTAC AACCAGGGAA TTGGAACCAA GTCATCATAT
301      ATATACATGT CCTGGGCAGG GCATCTGGAA GCCCAGGGAG AGCTGCAGCA TGCCAGTGCT
361      ATTTTTCAGA CAGGAATTCA CAATGAGGCT GAACCTAAAG AACTACTACA GCAACAATAC
421      AGGCTATTCC AAGCACGCCT TACTGGAATC CATTTGCCAG CTCAAGCTAC AACCTCAGAA
481      CCTTTGCATA GTGCACAGAT TTTAAACCAA GTTATGATGA CAAACTCAAG TCCAGAAAAA
541      AACTCAGCCT GTGTTCCTAA GAGTCAGGGT TCAGAATGTT CTGGTGTGGC ATCTTCCACT
601      TGTGATGAAA AGTCTAATAT AGAACAAAGG GTGATCATGA TTTCCAAGTC AGAATGCTCT
661      GTCAGCTCAT CTGTGGCACC CAAGCCTGAG GCTCAGCAAG TTATGTACTG CAAGGAAAAG
721      CTTATTCGTG GAGATTCAGA ATTTTCTTTT GAAGAACTGA GAGCCCAGAA ATATAATCAA
781      AGGAAGAAGC ATGAGCAGTG GGTTAGTGAA GACAGAAATT ATATGAAAAG GAAAGAAGCA
841      AATGCTTTTG AAGAGCAATT ATTAAAACAG AAAATGGATG AACTTCACAA GAAATTGCAT
901      CAAGTGGTGG AATTGTCACA CAAGGACCTT CCTGCTTCTG AGAACAGGCC TGATGTTAGT
961      CTAGTATGTG TTGGACAAAA TACTTGCTCC CAGCAGGAAT TGAGGGGTCC AAGTCTTTCA
1021     TCCATCAGTC ATCAGACCTC AGAGAGTTCA GGAGAGAAAC CACAGGAAGA ACCTTCTGTT
1081     CCTCTTATGG TAAATGCTGT TAACAGCACT TTGCTGTTCC CAGCTGCCAA CCTGCCAGCT
1141     CTTCCTGTTC CTGTAAGTGG CCAGTCATTG ACAGACTCCA GATGTGTGAA TCAAAGTGTT
1201     CATGAATTCA TGCCACAGTG TGGACCAGAA ACAAAAGAAG TGTGTGAAAC AAATAAAGTT
1261     GCCAGCATTA ATGATTTTCA TACAACTCCA AACACATCAT TGGGAATGGT TCAAGGAACA
1321     CCATGCAAAG TGCAGCCATC ACCAACTGTC CACACCAAGG AAGCATTAGG TTTCATCATG
1381     GACATGTTTC AGGCTCCAAC ACTTCCTGAC ATTTCTGATG ATAAAGATGA ATGGCCATCT
1441     CTGGACCAAA ATGAAGATGC ATTTGAAGCC CAGTTTCAAA AAAATGCAGT ATCTTCGGGA
1501     GATTGGGGAG TTAAAAAAAT TATGACTTTG TCATCTGCTT TTCCTATTTT TGAAGATGGA
1561     AACAAAGAAA ATTATGGCTT ACCACAGCCT AAAAATAAGC CCTTAGGAGC TAGGACCTTT
1621     GGAGAACGAT CTCTCAGTAA ATATTCCTCG AGATCAAATG AAATGCCTCA CACTGATGAG
1681     TTTATGGATG ATTCAACAGT ATGTGGTATT CGCTGCAACA AAACTCTAGC TCCCAGTCCT
1741     AAAAGTATAG GAGACTTTAC ATCTGCTGCC CAACTTTCGT CTACACCATT CCACAAATTT
1801     CCAGCAGATT TAGTACAGAT TCCAGAAGAT AAAGAAAATG TGGTAGCCAC ACAGTATACA
1861     CATATGGCTT TGGATTCTTG TAAAGAAAAC ATAGTGGACC TCTCAAAAGG CAGAAAGCTT
1921     GGGCCAATTC AAGAGAAAAT TTCAGCATCT TTACCCTGTC CTAGTCAGCC TGCCACAGGT
1981     GGTTTGTTCA CCCAGGAAGC AGTGTTCGGC CTTGAGGCTT TTAAATGCAC AGGCATTGAC
2041     CATGCGACAG TGGAAGACCT ATCCGATGCC AATGCTGGGC TCCAAGTTGA ATGCGTGCAG
2101     ACACTTGGAA ATGTCAATGC TCCAAGCTTT ACTGTTGAGA ACCCATGGGA TGATGAATTG
2161     ATTCTTAAAC TTCTCTCTGG ACTTTCTAAG CCAGTTACTT CCTATTCAAA TACTTTTGAG
2221     TGGCAGAGTA AACTTCCAGC CATCAAGACC AAGACAGAAT ATCAATTGGG TTCTTTGCTG
2281     GTCTATGTGA ATCACCTTCT TGGAGAAGGA GCCTTTGCTC AAGTCTTTGA AGCTATTCAT
2341     GGAGATGTGA GAAATGCCAA AAGTGAACAG AAATGCATTT TGAAGGTGCA GAGACCTGCC
2401     AACTCCTGGG AATTCTACAT TGGGATGCAG CTGATGGAAA GACTAAAGCC AGAAGTACAT
2461     CACATGTTCA TCAAGTTTTA TTCTGCTCAT TTATTCAAGA ACGGCAGCAT ATTAGTAGGG
2521     GAACTCTACA GCTATGGGAC GTTACTAAAT GTCATTAACC TCTATAAAAA TACCTCTGAA
2581     AAAGTGATGC CCCAGGCTCT TGTCCTCACT TTCGCTATCA GAATGCTTTA CATGGTTGAA
2641     CAAGTCCACA GCTGCGAAAT CATTCATGGA GACATTAAGC CAGATAACTT CATACTAGGA
2701     CACAGATTTT TGGAACAGGC TGATGAAGAC TTAGCTACCG GCTTGGCATT GATTGACCTG
2761     GGTCAGAGTA TAGATATGAA ACTTTTCCCT AAAGGAACTG TATTTACAGG AAAATGTGAA
2821     ACATCTGGTT TTCAGTGTCC TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT
2881     TACTTTGGAG TTGCTGCAAC AATATACTGT ATGCTCTTTG GCTCTTACAT GAAAGTAAAA
2941     AATGAAGGAG GAGTCTGGAA ACCTGAAGGT CTTTTTAGAA GGCTTCCTCA TTTGGATATG
3001     TGGGAGGAAT TTTTTCACAT CATGTTGAAT ATACCGGATT GTCATAATCT TCCATCTTTG
3061     GATTTTCTGA GACAGAATAT GAAGAAATTA CTTGAACAAC AGTATTCCAA CAAGATTAAG
3121     ACCTTGCGTA ATAGGCTAAT TGTGATGCTT TCAGAATATA AGCGTTCAAG AAAATAA

 

Quality Control:cDNA sequencing results is available upon request.