BTLA Protein, Mouse, Recombinant (Fc Tag)

Cat: 51060-M02H
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BTLA Protein, Mouse, Recombinant (Fc Tag) Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Immobilized mouse BTLA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated mouse HVEM-Fch (Cat:10567-M03S), The EC50 of biotinylated mouse HVEM-Fch (Cat:10567-M03S) is 15.7-36.7 ng/ml.
Protein Construction
A DNA sequence encoding the mouse BTLA (Met1-Gly176) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Glu 30
Molecule Mass
The recombinant mouse BTLA /Fc is a disulfide-linked homodimer. The reduced monomer comprises 388 amino acids and has a predicted molecular mass of 437 kDa. The apparent molecular mass of the protein is approximately 57-62 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
BTLA Protein, Mouse, Recombinant (Fc Tag) in SDS-PAGE
Mouse BTLA/CD272 Protein 12047
BTLA Protein, Mouse, Recombinant (Fc Tag) Alternative Names
A630002H24 Protein, Mouse
BTLA Background Information

BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).

Immune Checkpoint
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
B and T lymphocyte associated
References
  • Fourcade J, et al. (2012) CD8(+) T cells specific for tumor antigens can be rendered dysfunctional by the tumor microenvironment through upregulation of the inhibitory receptors BTLA and PD-1. Cancer Res. 72(4):887-96.
  • Kojima R, et al. (2011) Molecular basis for herpesvirus entry mediator recognition by the human immune inhibitory receptor CD160 and its relationship to the cosignaling molecules BTLA and LIGHT. J Mol Biol. 413(4):762-72.
  • Oki M, et al. (2011) A functional polymorphism in B and T lymphocyte attenuator is associated with susceptibility to rheumatoid arthritis. Clin Dev Immunol. 305656.
  • Other BTLA Proteins

    Rhesus BTLA Proteins

    Product Name Cat.No.
    BTLA Protein, Rhesus, Recombinant (Fc Tag) 90250-C02H
    BTLA Protein, Rhesus, Recombinant (His Tag) 90250-C08H

    Rat BTLA Proteins

    Product Name Cat.No.
    BTLA Protein, Rat, Recombinant (ECD, Fc Tag) 81142-R05H

    Human BTLA Proteins

    Product Name Cat.No.
    BTLA Protein, Human, Recombinant (Fc Tag) 11895-H02H
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