Mouse BST2 Gene cDNA Clone / ORF Clone

BST2 cDNA Clone Datasheet

BST2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_198095.2
• cDNA Size: 540
• Gene Synonym: GREG; Bst-2; C87040; DAMP-1; 2310015I10Rik; Bst2
• Source: Mouse

BST2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus bone marrow stromal cell antigen 2 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 517 C/T resulting in the amino acid Pro substitution by Ser.

BST2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BST2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BST2 cDNA Clone Sequence and Quality Control

Sequence:

1        GACGGATCAC ATGGCGCCCT CTTTCTATCA CTATCTGCCC GTGCCCATGG ATGAGATGGG
61       GGGGAAGCAA GGATGGGGCA GCCACCGGCA GTGGCTGGGG GCCGCGATCT TGGTGGTCCT
121      GTTCGGGGTT ACCTTAGTCA TCCTGACAAT CTACTTCGCC GTCACAGCGA ACAGCGTGGC
181      CTGTAGAGAC GGGTTGCGAG CGCAGGCTGA GTGCCGGAAC ACCACGCACC TGTTGCAGCG
241      CCAGCTCACC CGCACCCAGG ACAGTCTGCT GCAGGCCGAG ACACAGGCAA ACTCCTGCAA
301      CCTGACCGTG GTGACCCTTC AGGAGTCCCT GGAGAAGAAG GTGTCTCAAG CCCTGGAGCA
361      GCAGGCCCGC ATCAAGGAGC TTGAGAATGA AGTCACGAAG CTGAACCAGG AGCTGGAGAA
421      TCTGAGGATC CAAAAGGAGA CTTCTAGCAC AGTGCAGGTG AACTCTGGCA GCTCCATGGT
481      GGTCTCCAGC CTACTGGTGC TCAAAGTGTC ACTGTTTCTG CTCTTTTGAG GACTCATTAG

 

Quality Control:cDNA sequencing results is available upon request.