Rat BST1 Gene cDNA Clone / ORF Clone

BST1 cDNA Clone Datasheet

BST1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_030848.1
• cDNA Size: 960
• Gene Synonym: Bst1
• Source: Rat

BST1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus bone marrow stromal cell antigen 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 705 T/C not causing the amino acid variation.

BST1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BST1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BST1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGTTC AAGCATGCGC GTTGTCTCTA CGGCTGGGGC TGTGGATGTC CCTCTTGCTG
61       CCGGTACTGC CGGGGGCGGG GGCGAGAGCT GCTGGAGCGC GGTGGAGCGG GGAGGGTACC
121      ACGCCTCACC TCCAGAGCAT CTTCCTGGGC CGCTGTGCGG AGTACACCAC GCTGCTGAGC
181      CTTGAGCCGG GGAACAAAAA CTGCACAGCC ATCTGGGAGG CCTTCAAGGT GGTGCTGGAC
241      AAGGATCCCT GCTCTGTGCT CCCCTCAGAC TATGATCTCT TCATTAACCT GTCCAGGCAC
301      GCCATTCCTA GGGACAAGTC ACTGTTCTGG GAAAATAACC ACCTACTGGT TATGAGCTAT
361      GCAGAGAACA CCCGCCGCCT TATGCCCCTG TGTGACGTTC TGTATGGCAA GGTTGGAGAT
421      TTCTTGAGCT GGTGTCGACA GGAAAATGCC TCTGGACTTG ATTACCAGTC CTGCCCCACA
481      GCAGAAGACT GTGAAAACAA TGCCGTGGAT GCCTATTGGA AAAGTGCATC CATGCAGTAT
541      TCAAGAGACA GTTCAGGAGT GATCAACGTC ATGCTGAATG GCTCGGAGCC AAAAGGAGCC
601      TATCCCACGA AAGGGTTTTT TGCAGATTTT GAAATCCCAT ACTTGCAAAA GGATAAAATC
661      ACAAGAATTG AGATCTGGGT CATGCATGAA GTTGGGGGAC CCCACGTGGA ATCCTGCGGC
721      GAAGGCAGTG TTAAGATCCT GGAAGACAGA CTGGAGGCCT TGGGGTTCCA GCACAGCTGT
781      ATCAATGACT ACCCGCCGGT GAAGTTCTTA ATGTGTGTGG ACCACAGCAC TCACCCCGAC
841      TGTGCCATGA ACTCAGCCTC AGCGTCTATG TGGAGGGAGA GCCCAGCTCT TCATGCAATA
901      GGAGACATCA GCCTCATCAT CTCTCTCTTA GTGGCTTTGG CTTCAAGTTC TCAAGCATGA

 

Quality Control:cDNA sequencing results is available upon request.