Human BRSK1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12322-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

BRSK1 cDNA Clone Datasheet

BRSK1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC016681
cDNA Size:	1422
Gene Synonym:	hSAD1；BRSK1
Source:	Human

BRSK1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens BR serine/threonine kinase 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

BRSK1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BRSK1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BRSK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGAGCC TGCCATCCAA CGGAGAGCTG GACCCCGACG TCCTAGAGAG CATGGCATCA
61       CTGGGCTGCT TCAGGGACCG CGAGAGGCTG CATCGCGAGC TGCGCAGTGA GGAGGAGAAC
121      CAAGAAAAGA TGATATATTA TCTGCTTTTG GATCGGAAGG AGCGGTATCC CAGCTGTGAG
181      GACCAGGACC TGCCTCCCCG GAATGATGTT GACCCCCCCC GGAAGCGTGT GGATTCTCCC
241      ATGCTGAGTC GTCACGGGAA GCGGCGACCA GAGCGGAAGT CCATGGAAGT CCTGAGCATC
301      ACCGATGCCG GGGGTGGTGG CTCCCCTGTA CCCACACGAC GGGCCTTGGA GATGGCCCAG
361      CACAGCCAGA GATCCCGTAG CGTCAGTGGA GCCTCCACGG GTCTGTCCTC CAGCCCTCTA
421      AGCAGCCCAA GGAGTCCGGT CTTTTCCTTT TCACCGGAGC CGGGGGCTGG AGATGAGGCT
481      CGAGGCGGGG GCTCCCCGAC TTCCAAAACG CAGACGCTGC CTTCTCGGGG CCCCAGGGGT
541      GGGGGCGCCG GGGAGCAGCC CCCGCCCCCC AGTGCCCGCT CCACACCCCT GCCCGGCCCC
601      CCAGGCTCCC CGCGCTCCTC TGGCGGGACC CCCTTGCACT CGCCTCTGCA CACGCCCCGG
661      GCCAGTCCCA CCGGGACCCC GGGGACAACA CCACCCCCCA GCCCCGGCGG TGGCGTCGGG
721      GGAGCCGCCT GGAGGAGTCG TCTCAACTCC ATCCGCAACA GCTTCCTGGG CTCCCCTCGC
781      TTTCACCGGC GCAAGATGCA GGTCCCTACC GCTGAGGAGA TGTCCAGCTT GACGCCAGAG
841      TCCTCCCCGG AGCTGGCAAA ACGCTCCTGG TTCGGGAACT TCATCTCCTT GGACAAAGAA
901      GAACAAATAT TCCTCGTGCT AAAGGACAAA CCTCTCAGCA GCATCAAAGC AGACATCGTC
961      CATGCCTTTC TGTCGATCCC CAGCCTGAGT CACAGTGTGC TGTCACAGAC CAGCTTCAGG
1021     GCCGAGTACA AAGCCAGTGG CGGCCCCTCC GTCTTCCAAA AGCCCGTCCG CTTCCAGGTG
1081     GACATCAGCT CCTCTGAGGG TCCAGAGCCC TCCCCGCGAC GGGACGGCAG CGGAGGTGGT
1141     GGCATCTACT CCGTCACCTT CACTCTCATC TCGGGTCCCA GCCGTCGGTT CAAGCGAGTG
1201     GTGGAGACCA TCCAGGCACA GCTCCTGAGC ACTCATGACC AGCCCTCCGT GCAGGCCCTG
1261     GCAGACGAGA AGAACGGGGC CCAGACCCGG CCTGCTGGTG CCCCACCCCG AAGCCTGCAG
1321     CCCCCACCCG GCCGCCCAGA CCCAGAGCTG AGCAGCTCTC CCCGCCGAGC CCCCCCCAAG
1381     GACAAGAAGC TCCTGGCCAC CAACGGGACC CCTCTGCCCT GA

Quality Control:cDNA sequencing results is available upon request.