Human BOLA1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13743-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

BOLA1 cDNA Clone Datasheet

BOLA1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC063405
cDNA Size:	414
Gene Synonym:	CGI-143; MGC75015; RP11-196G18.18; hBolA; BOLA1
Source:	Human

BOLA1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens bolA homolog 1 (E. coli) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

BOLA1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BOLA1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BOLA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGAGTG GGCGGCTGGT CCTGGGTCTG GTCTCCATGG CTGGCCGCGT TTGTTTGTGC
61       CAGGGCAGCG CGGGATCCGG GGCCATCGGT CCGGTGGAGG CCGCCATTCG CACGAAGTTG
121      GAGGAGGCCC TGAGCCCCGA GGTGCTAGAG CTTCGCAACG AGAGCGGTGG CCACGCGGTC
181      CCGCCTGGCA GTGAGACTCA CTTCCGCGTG GCTGTGGTGA GCTCTCGTTT CGAGGGACTG
241      AGCCCCCTAC AACGACACCG GCTGGTCCAC GCAGCGCTGG CCGAGGAGCT GGGAGGTCCG
301      GTCCATGCGC TGGCCATCCA GGCACGGACC CCCGCCCAGT GGAGAGAGAA CTCTCAGCTG
361      GACACTAGCC CCCCATGCCT GGGTGGGAAC AAGAAAACTC TAGGAACCCC CTGA

Quality Control:cDNA sequencing results is available upon request.