|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BMP-7 ORF mammalian expression plasmid, C-GFPSpark tag||HG10083-ACG|
|Human BMP-7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10083-ACR|
|Human BMP-7 ORF mammalian expression plasmid, C-Flag tag||HG10083-CF|
|Human BMP-7 ORF mammalian expression plasmid, C-His tag||HG10083-CH|
|Human BMP-7 ORF mammalian expression plasmid, C-Myc tag||HG10083-CM|
|Human BMP-7 ORF mammalian expression plasmid, C-HA tag||HG10083-CY|
|Human BMP-7 natural ORF mammalian expression plasmid, HA tag||HG10083-M-Y|
|Human BMP-7 ORF mammalian expression plasmid, N-Flag tag||HG10083-NF|
|Human BMP-7 ORF mammalian expression plasmid, N-His tag||HG10083-NH|
|Human BMP-7 ORF mammalian expression plasmid, N-Myc tag||HG10083-NM|
|Human BMP-7 ORF mammalian expression plasmid, N-HA tag||HG10083-NY|
|Human BMP-7 natural ORF mammalian expression plasmid||HG10083-UT|
|Learn more about expression Vectors|
BMP-7 (Bone morphogenetic protein 7), also known as osteogenic protein 1 (OP-1), is a bone morphogenetic protein which belongs to the TGF-β superfamily. OP-1 is expressed in the brain, kidneys and bladder. BMP-7 may be involved in bone homeostasis. Osteogenic protein 1 plays a key role in the transformation of mesenchymal cells into bone and cartilage. The phosphorylation of SMAD1 and SMAD5 can be induced by BMP-7, which in turn induce transcription of numerous osteogenic genes. BMP-7 treatment can also induce all of the genetic markers of osteoblast differentiation in many cell types. The expression of BMP-7 causes ventral phenotypes while its complete inhibition creates a dorsal phenotype. Human recombinant BMP-7 protein can be used to aid in the fusion of vertebral bodies to prevent neurologic trauma. It also functions in the treatment of tibial non-union, frequently in cases where a bone graft has failed. It is found that BMP7 has the potential for treatment of chronic kidney disease.