BMP-7 Antibody (Cytokine)

BMP-7 Antibody Datasheet

BMP-7 Antibody Product Information

• Immunogen: Recombinant Human BMP-7 protein
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 02
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-7 (rh BMP-7). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BMP-7 Antibody Usage Guide

• Specificity: Human BMP-7
• No cross-reactivity in ELISA with
  Human cell lysate (293 cell line)
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human BMP7. The detection limit for Human BMP7 is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

BMP-7 Antibody Background

BMP-7 (Bone morphogenetic protein 7), also known as osteogenic protein 1 (OP-1), is a bone morphogenetic protein which belongs to the TGF-β superfamily. OP-1 is expressed in the brain, kidneys and bladder. BMP-7 may be involved in bone homeostasis. Osteogenic protein 1 plays a key role in the transformation of mesenchymal cells into bone and cartilage. The phosphorylation of SMAD1 and SMAD5 can be induced by BMP-7, which in turn induce transcription of numerous osteogenic genes. BMP-7 treatment can also induce all of the genetic markers of osteoblast differentiation in many cell types. The expression of BMP-7 causes ventral phenotypes while its complete inhibition creates a dorsal phenotype. Human recombinant BMP-7 protein can be used to aid in the fusion of vertebral bodies to prevent neurologic trauma. It also functions in the treatment of tibial non-union, frequently in cases where a bone graft has failed. It is found that BMP7 has the potential for treatment of chronic kidney disease.

References

1. Shi J. et al., 2010, Fertil Steril. 93(4): 1273-9.
2. González EA. et al., 2002, Kidney Int. 61(4): 1322-31.
3. Gould SE. et al., 2002, Kidney Int. 61(1): 51-60.
4. Hahn GV. et al., 1992, Genomics. 14(3): 759-62.