|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human BMP-2 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG10426-G-F|
|Human BMP-2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG10426-G-H|
|Human BMP-2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG10426-G-N|
|Human BMP-2 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG10426-G-Y|
BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.