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Product Catalog
BMP-2 ELISA Pair Set, Human ELISA Pair Set (Cytokine) 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10426 |
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Human BMP-2 ELISA Pair Set
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Order or Inquire for BMP-2 ELISA Pair Set |
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High affinity Human BMP-2 ELISA Pair Set |
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Detection limit - 78 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human BMP-2 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Bone morphogenetic protein 2 (BMP2) is a member of the BMP subgroup belonging to the transforming growth factor (TGF)-beta superfamily. The mature BMP2 acts as disulfide-linked homodimers and heterodimers with BMP-7 after proteolytic removal of propeptide. BMP2 is capable of inducing the formation of cartilage and bone but are now regarded as multifunctional cytokines. It binds heterodimeric receptor complexes composed of a type I receptor and a type I I receptor, and transduces the related signals via the down-stream molecules, such as the PI3 kinase, Smads, STATs, etc. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function. In addition, recent studies have revealed that osteoblast-derived BMP2 enhances the migration of prostate cancer cells. rhBMP2 can be utilized in various therapeutic interventions such as bony defects, delayed union, non-union fractures and osteoporosis.
