BMP-2 Antibody

BMP-2 Antibody Datasheet

BMP-2 Antibody Product Information

• Immunogen: Recombinant Human BMP-2 protein (Catalog#10426-HNAE)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 7D10G8E2
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-2 (rh BMP-2; Catalog#10426-HNAE; NP_001191.1; Gln 283-Arg 396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BMP-2 Antibody Usage Guide

• Specificity: Human BMP-2
• No cross-reactivity in ELISA with

  Human BMP5

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human BMP2 in WB. Using a DAB detection system, the detection limit for Human BMP2 is approximately 2 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human BMP2. The detection limit for Human BMP2 is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

BMP-2 Antibody Background

Bone morphogenetic protein 2 (BMP2) is a member of the BMP subgroup belonging to the transforming growth factor (TGF)-beta superfamily. The mature BMP2 acts as disulfide-linked homodimers and heterodimers with BMP-7 after proteolytic removal of propeptide. BMP2 is capable of inducing the formation of cartilage and bone but are now regarded as multifunctional cytokines. It binds heterodimeric receptor complexes composed of a type I receptor and a type II receptor, and transduces the related signals via the down-stream molecules, such as the PI3 kinase, Smads, STATs, etc. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function. In addition, recent studies have revealed that osteoblast-derived BMP2 enhances the migration of prostate cancer cells. rhBMP2 can be utilized in various therapeutic interventions such as bony defects, delayed union, non-union fractures and osteoporosis.

References

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