Anti-BMP-2 Antibody

Cat: 10426-MM14
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All BMP-2 Reagents
Anti-BMP-2 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-BMP-2 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human BMP-2
No cross-reactivity in ELISA with Human BMP5 E.coli cell lysate
Recombinant Human BMP-2 protein (Catalog#10426-HNAE)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-2 (rh BMP-2 ; Catalog#10426-HNAE; NP_001191.1; Gln 283-Arg 396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #7D10G8E2
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BMP-2 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BMP-2 Antibody (Mouse Monoclonal antibody) Images
Human BMP-2 Immunoprecipitation(IP) 6163

BMP2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg Caco-2 Whole Cell Lysate

0.5 µL anti-BMP2 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-BMP2 mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size: 58 kDa

Human BMP-2 Western blot (WB) 16047

Anti-BMP2 mouse monoclonal antibody at 1:500 dilution

Lane A: Caco2 Whole Cell Lysate

Lane B: THP1 Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:39 kDa

(We are unsure as to the identity of these extra bands.)

Anti-BMP-2 Antibody Alternative Names
Anti-BDA2 Antibody;Anti-BDA2A Antibody;Anti-BMP-2 Antibody;Anti-BMP2A Antibody
BMP-2 Background Information

BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

Full Name
bone morphogenetic protein 2
  • Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.
  • Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BMP-2 Antibody Mouse Monoclonal ELISA(Det) 10426-MM03
    Anti-BMP-2 Antibody Rabbit Monoclonal ICC/IF,IF 10426-R110
    Anti-BMP-2 Antibody Mouse Monoclonal ICC/IF,IF 10426-MM22
    Anti-BMP-2 Antibody Mouse Monoclonal ELISA(Cap) 10426-MM23
    Anti-BMP-2 Antibody Rabbit Polyclonal ELISA 10426-T16
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