Anti-BMP-2 Antibody

Cat: 10426-MM14
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All BMP-2 Reagents
Anti-BMP-2 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-BMP-2 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human BMP-2
No cross-reactivity in ELISA with Human BMP5 E.coli cell lysate
Immunogen
Recombinant Human BMP-2 protein (Catalog#10426-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-2 (rh BMP-2 ; Catalog#10426-HNAE; NP_001191.1; Gln 283-Arg 396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #7D10G8E2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BMP-2 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BMP-2 Antibody (Mouse Monoclonal antibody) Images
Human BMP-2 Immunoprecipitation(IP) 6163

BMP2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg Caco-2 Whole Cell Lysate

0.5 µL anti-BMP2 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-BMP2 mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size: 58 kDa

Human BMP-2 Western blot (WB) 16047

Anti-BMP2 mouse monoclonal antibody at 1:500 dilution

Lane A: Caco2 Whole Cell Lysate

Lane B: THP1 Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:39 kDa

(We are unsure as to the identity of these extra bands.)

Anti-BMP-2 Antibody Alternative Names
Anti-BDA2 Antibody;Anti-BDA2A Antibody;Anti-BMP-2 Antibody;Anti-BMP2A Antibody
BMP-2 Background Information

BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

Full Name
bone morphogenetic protein 2
References
  • Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.
  • Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BMP-2 Antibody Mouse Monoclonal ELISA(Det) 10426-MM03
    Anti-BMP-2 Antibody Rabbit Monoclonal ICC/IF,IF 10426-R110
    Anti-BMP-2 Antibody Mouse Monoclonal ICC/IF,IF 10426-MM22
    Anti-BMP-2 Antibody Mouse Monoclonal ELISA(Cap) 10426-MM23
    Anti-BMP-2 Antibody Rabbit Polyclonal ELISA 10426-T16
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