|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
BMP2 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg Caco-2 Whole Cell Lysate0.5 µL anti-BMP2 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-BMP2 mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 58 kDa
Observed band size: 58 kDa
Anti-BMP2 mouse monoclonal antibody at 1:500 dilution
Lane A: Caco2 Whole Cell Lysate
Lane B: THP1 Whole Cell Lysate
Lane C: HeLa Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:45 kDa
Observed band size:39 kDa
(We are unsure as to the identity of these extra bands.)
BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|