Anti-BLVRB Antibody

Cat: 13151-R009
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Anti-BLVRB Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-BLVRB Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human BLVRB
Immunogen
Recombinant Human BLVRB / biliverdin reductase B protein (Catalog#13151-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human BLVRB / biliverdin reductase B (rh BLVRB / biliverdin reductase B; Catalog#13151-H07E; P30043; Ala2-Gln206).
Source
Monoclonal Rabbit IgG Clone #009
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BLVRB Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BLVRB Antibody (Rabbit Monoclonal antibody) Images
Human BLVRB Western blot (WB) 13538

Anti-BLVRB rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:22 kDa

Human BLVRB Immunoprecipitation(IP) 17709

BLVRB was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

Lane C:0.5 mg K562 Whole Cell Lysate

Lane D:0.5 mg Caco-2 Whole Cell Lysate

2 µL anti-BLVRB rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-BLVRB rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 22 kDa

Observed band size: 22 kDa

Anti-BLVRB Antibody Alternative Names
Anti-BVRB Antibody;Anti-FLR Antibody;Anti-HEL-S-10 Antibody;Anti-SDR43U1 Antibody
BLVRB Background Information

Biliverdin reductase (hBVR) is a serine/threonine kinase that catalyzes reduction of the heme oxygenase (HO) activity product, biliverdin, to bilirubin. BVR consists of an N-terminal dinucleotide-binding domain (Rossmann-fold) and a C-terminal domain that contains a six-stranded β-sheet that is flanked on one face by several α-helices. The C-terminal and N-terminal domains interact extensively, forming the active site cleft at their interface. Biliverdin reductase (BVR) catalyzes the last step in heme degradation by reducing the γ-methene bridge of the open tetrapyrrole, biliverdin IXα, to bilirubin with the concomitant oxidation of a β-nicotinamide adenine dinucleotide (NADH) or β-nicotinamide adenine dinucleotide phosphate (NADPH) cofactor. It is now recognized that human BVR (hBVR) is a dual-specificity kinase (Ser / Thr and Tyr) upstream activator of the insulin/insulin growth factor-1 (IGF-1) and mitogen-activated protein kinase (MAPK) signaling pathways. Human BVR (hBVR) is essential for MAPK-extracellular signal-regulated kinase (ERK)1/2 (MEK)-eukaryotic-like protein kinase (Elk) signaling and has been identified as the cytoplasm-nuclear heme transporter of ERK1/2 and hematin, the key components of stress-responsive gene expression.

Full Name
biliverdin reductase B
References
  • Kapitulnik J, et al. (2009) Pleiotropic functions of biliverdin reductase: cellular signaling and generation of cytoprotective and cytotoxic bilirubin. Trends in Pharmacological Sciences. 30(3): 129-37.
  • Ahmad Z, et al. (2002) Human Biliverdin Reductase Is a Leucine Zipper-like DNA-binding Protein and Functions in Transcriptional Activation of Heme Oxygenase-1 by Oxidative Stress. The Journal of Biological Chemistry. 277: 9226-32.
  • Whitby FG, et al. (2002) Crystal Structure of a Biliverdin IX Reductase Enzyme-Cofactor Complex. Journal of Molecular Biology. 319(5): 1199-210.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BLVRB Antibody Mouse Monoclonal WB 13151-MM08
    Anti-BLVRB Antibody Rabbit Polyclonal ELISA 13151-RP01
    Anti-BLVRB Antibody Rabbit Polyclonal WB,ELISA,IP 13151-RP02
    Anti-BLVRB Antibody Mouse Monoclonal ELISA 13151-MM02
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