Anti-BLNK Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human BLNK / Ly-57 / SLP-65 protein (Catalog#12706-H07H)
Produced in rabbits immunized with purified, recombinant Human BLNK / Ly-57 / SLP-65 (rh BLNK / Ly-57 / SLP-65; Catalog#12706-H07H; P08195-1; Met 1-Ser 456). BLNK / Ly-57 / SLP-65 specific IgG was purified by Human BLNK / Ly-57 / SLP-65 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BLNK Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-BLNK Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of human BLNK in human tonsil with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human BLNK in human spleen with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human BLNK in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Anti-BLNK rabbit polyclonal antibody at 1:500 dilution
Lane A: Raji Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:50 kDa
Observed band size:70 kDa
Anti-BLNK Antibody Alternative Names
Anti-AGM4 Antibody;Anti-BASH Antibody;Anti-bca Antibody;Anti-BLNK-S Antibody;Anti-LY57 Antibody;Anti-SLP-65 Antibody;Anti-SLP65 Antibody
BLNK Background Information
B-cell linker protein, also known as B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65 and BLNK, is a cytoplasm and cell membrane protein which contains one SH2 domain. BLNK is expressed in B-cell lineage and fibroblast cell lines. Highest levels of expression is in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. BLNK functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. BLNK plays a role in the activation of ERK / EPHB2, MAP kinase p38 and JNK. BLNK modulates AP1 activation. It is important for the activation of NF-kappa-B and NFAT. BLNK plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK may be required for the RAC1-JNK pathway. It plays a critical role in orchestrating the pro-B cell to pre-B cell transition. BLNK also plays an important role in BCR-induced B-cell apoptosis. Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) which is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development.
Fu C., et al., 1998, Immunity 9:93-103. Minegishi Y., et al., 1999, Science 286:1954-1957. Chiu C.W., et al., 2002, EMBO J. 21:6461-6472. Taguchi T., et al., 2004, Immunology 112:575-582.