Anti-BLMH Antibody

Cat: 11333-RP02
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Anti-BLMH Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-BLMH Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human BLMH
Immunogen
Recombinant Human BLMH / Bleomycin Hydrolase protein (Catalog#11333-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human BLMH / Bleomycin Hydrolase (rh BLMH / Bleomycin Hydrolase; Catalog#11333-H07E; NP_000377.1; Ser 2-Glu 455). BLMH / Bleomycin Hydrolase specific IgG was purified by Human BLMH / Bleomycin Hydrolase affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BLMH Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BLMH Antibody (Rabbit Polyclonal antibody) Images
Human BLMH/Bleomycin Hydrolase Immunoprecipitation(IP) 8084

BLMH was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-BLMH rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-BLMH rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 53 kDa

Observed band size: 53 kDa

Human BLMH/Bleomycin Hydrolase Western blot (WB) 13394

Anti-BLMH rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:53 kDa

Observed band size:53 kDa

Anti-BLMH Antibody Alternative Names
Anti-BH Antibody;Anti-BMH Antibody
BLMH Background Information

The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.

Full Name
bleomycin hydrolase
Research Areas
References
  • Takeda A, et al. (1996) Cloning and Analysis of cDNA Encoding Rat Bleomycin Hydrolase, a DNA-Binding Cysteine Protease. J Biochem. 120 (2): 353-9.
  • Lefterov IM, et al. (2000) Human bleomycin hydrolase regulates the secretion of amyloid precursor protein. The FASEB Journal. 14(12): 1837-47.
  • Brmme D, et al. (1996) Human Bleomycin Hydrolase: Molecular Cloning, Sequencing, Functional Expression, and Enzymatic Characterization. Biochemistry. 35 (21): 6706-14.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BLMH Antibody Rabbit Polyclonal ELISA 50668-RP01
    Anti-BLMH Antibody Rabbit Polyclonal WB,ELISA,IP 50668-RP02
    Anti-BLMH Antibody Rabbit Polyclonal ELISA 11333-RP01
    info info
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