BID Matched ELISA Antibody Pair Set,Human

Cat: SEK10468
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BID Matched ELISA Antibody Pair Set,Human General Information
Product name
BID Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human BID
Assay range
7.81-500
Materials provided
1. Capture Antibody 0.5 mg/mL of mouse anti-BID monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10468-MM10)
2. Detection Antibody 1.0 mg/mL mouse anti-BID monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10468-MM02)
3. Standard Each vial contains 17 ng of recombinant BID. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended.
Product overview
This BID Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human BID . It contains Human BID capture antibody, Human BID detector antibody (HRP) and a highly purified E. coli-expressed recombinant Human BID protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
BID Matched ELISA Antibody Pair Set,Human Images
Human p22 BID ELISA Pair Set Standard Curve 13843
BID Matched ELISA Antibody Pair Set,Human Alternative Names
FP497 Matched ELISA Antibody Pair Set, Human
BID Background Information

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Full Name
BH3 interacting domain death agonist
References
  • Gross A. (2006) BID as a double agent in cell life and death. Cell Cycle. 5(6): 582-4.
  • Yin XM. (2007) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.
  • Esposti MD. (2002) The roles of Bid. Apoptosis. 7(5): 433-40.
  • Yin XM. (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10(3): 161-7.
  • Yin XM. (2000) Bid, a critical mediator for apoptosis induced by the activation of Fas/TNF-R1 death receptors in hepatocytes. J Mol Med. 78(4): 203-11.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    BID ELISA Kit, Human Serum KIT10468
    info info
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