|ELISA(Det)||1:1000-1:10000||This antibody will detect Human p22 BID in ELISA pair set (Catalog: # SEK10468). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10468-MM10).|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-BID mouse monoclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:22 kDa
Observed band size:22 kDa
The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-BID Antibody (PE)||Mouse||Monoclonal||FCM||10468-MM10-P|
|Anti-BID Antibody (FITC)||Mouse||Monoclonal||FCM||10468-MM10-F|