Anti-BID Antibody (FITC)

Cat: 10468-MM10-F
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Anti-BID Antibody (FITC) (Mouse Monoclonal antibody) General Information
Product name
Anti-BID Antibody (FITC)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human BID
Immunogen
Recombinant Human BID protein (Catalog#10468-HNCE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BID (rh BID; Catalog#10468-HNCE; P55957-1; Met 1-Asp 195) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG1 Clone #10
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-BID Antibody (FITC) (Mouse Monoclonal antibody) Validated Applications

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BID Antibody (FITC) (Mouse Monoclonal antibody) Images
Human p22 BID Flow Cytometry (FC) 4858
Flow cytometric analysis of BID expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-BID. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-BID Antibody (FITC) Alternative Names
Anti-FP497 Antibody
BID Background Information

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Full Name
BH3 interacting domain death agonist
References
  • Gross A. (2006) BID as a double agent in cell life and death. Cell Cycle. 5(6): 582-4.
  • Yin XM. (2007) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.
  • Esposti MD. (2002) The roles of Bid. Apoptosis. 7(5): 433-40.
  • Yin XM. (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10(3): 161-7.
  • Yin XM. (2000) Bid, a critical mediator for apoptosis induced by the activation of Fas/TNF-R1 death receptors in hepatocytes. J Mol Med. 78(4): 203-11.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BID Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50351-T60
    Anti-BID Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 10468-T60
    Anti-BID Antibody Mouse Monoclonal WB,ELISA,ELISA(Cap),FCM,ICC/IF,IF 10468-MM10
    Anti-BID Antibody (PE) Mouse Monoclonal FCM 10468-MM10-P
    Anti-BID Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 10468-R005
    Anti-BID Antibody Rabbit Polyclonal WB 10468-RP01
    Anti-BID Antibody Rabbit Polyclonal WB,ELISA,IP 10468-RP02
    Anti-BID Antibody Mouse Monoclonal WB,ELISA,ELISA(Det) 10468-MM02
    Anti-BID Antibody Rabbit Monoclonal WB,ELISA,IP 10468-R002
    Anti-BID Antibody Rabbit Polyclonal ELISA,IP 10468-T20
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