Anti-BID Antibody

Cat: 10468-RP02
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Anti-BID Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-BID Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human BID
Immunogen
Recombinant Human BID protein (Catalog#10468-HNCE)
Preparation
Produced in rabbits immunized with purified, recombinant Human BID (rh BID; Catalog#10468-HNCE; P55957-1; Met 1-Asp 195). BID specific IgG was purified by Human BID affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BID Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BID Antibody (Rabbit Polyclonal antibody) Images
Human p22 BID Western blot (WB) 13231

Anti-BID rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:21 kDa

Human p22 BID Immunoprecipitation(IP) 17673

BID was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg RAW264.7 Whole Cell Lysate

Lane D:0.5 mg 293T Whole Cell Lysate

Lane E:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-BID rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-BID rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 22 kDa

Observed band size: 22 kDa

Anti-BID Antibody Alternative Names
Anti-FP497 Antibody
BID Background Information

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Full Name
BH3 interacting domain death agonist
References
  • Gross A. (2006) BID as a double agent in cell life and death. Cell Cycle. 5(6): 582-4.
  • Yin XM. (2007) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.
  • Esposti MD. (2002) The roles of Bid. Apoptosis. 7(5): 433-40.
  • Yin XM. (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10(3): 161-7.
  • Yin XM. (2000) Bid, a critical mediator for apoptosis induced by the activation of Fas/TNF-R1 death receptors in hepatocytes. J Mol Med. 78(4): 203-11.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BID Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50351-T60
    Anti-BID Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 10468-T60
    Anti-BID Antibody Mouse Monoclonal WB,ELISA,ELISA(Cap),FCM,ICC/IF,IF 10468-MM10
    Anti-BID Antibody (PE) Mouse Monoclonal FCM 10468-MM10-P
    Anti-BID Antibody (FITC) Mouse Monoclonal FCM 10468-MM10-F
    Anti-BID Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 10468-R005
    Anti-BID Antibody Rabbit Polyclonal WB 10468-RP01
    Anti-BID Antibody Mouse Monoclonal WB,ELISA,ELISA(Det) 10468-MM02
    Anti-BID Antibody Rabbit Monoclonal WB,ELISA,IP 10468-R002
    Anti-BID Antibody Rabbit Polyclonal ELISA,IP 10468-T20
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