Human BEST3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12148-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

BEST3 cDNA Clone Datasheet

BEST3 cDNA Clone Product Information

Gene Bank Ref.ID:	BC028087
cDNA Size:	807
Gene Synonym:	VMD2L3;MGC13168; MGC40411;BEST3
Source:	Human

BEST3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens bestrophin 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

BEST3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BEST3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BEST3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACAACAG ATGAAAGGAA ATTATTCAAC CACCTCAAGT CTCCTCATCT GAAATATTGG
61       GTTCCATTCA TCTGGTTTGG AAATCTTGCA ACTAAAGCCC GGAATGAAGG TAGAATCAGA
121      GACAGTGTTG ATCTGCAATC ATTGATGACT GAAATGAATC GATACCGCTC TTGGTGCAGC
181      CTCTTATTCG GTTATGACTG GGTTGGGATT CCGCTGGTTT ACACCCAGGT AGCAGAGCAG
241      CTTATCAACC CTTTTGGAGA AGATGATGAT GATTTTGAAA CTAACTGGTG CATTGACAGA
301      AATTTGCAGG TCTCTCTTTT AGCTGTGGAC GAAATGCACA TGAGCTTACC CAAGATGAAG
361      AAGGACATTT ACTGGGACGA TTCTGCTGCT CGCCCACCAT ACACATTGGC AGCTGCTGAC
421      TACTGCATAC CCTCATTTCT GGGGTCAACA GTCCAGATGG GGCTGTCTGG GTCCGACTTT
481      CCTGACGAGG AGTGGCTGTG GGATTATGAG AAGCATGGCC ATCGGCATTC CATGATAAGA
541      AGAGTCAAGC GGTTCCTGAG TGCCCACGAA CACCCCTCCA GCCCCAGAAG AAGAAGCTAC
601      AGGAGGCAGA CAAGTGACAG CTCCATGTTC TTACCCCGAG ATGACCTCAG CCCAGCCAGG
661      GACCTACTGG ATGTGCCCTC AAGAAACCCC CCCAGGGCCT CACCCACCTG GAAGAAATCC
721      TGCTTCCCAG AAGGAAGCCC CACGCTGCAC TTCAGCATGG GAGAGCTGTC CACCATCAGG
781      GAGACCAGCC AGACAAGCAC TTTATAG

Quality Control:cDNA sequencing results is available upon request.