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Human BDNF transcript variant 4 Gene cDNA Clone (full-length ORF Clone)
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Human BDNF transcript variant 4 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10068-M




  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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BDNF cDNA Clone Datasheet

BDNF cDNA Clone Product Information

Gene Bank Ref.ID: NM_001709.3

cDNA Size:

744

Gene Synonym:

BDNF, MGC34632

Source: Human

BDNF cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens brain derived neurotrophic factor DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description:

Identical with the Gene Bank Ref. ID sequence except for a point mutation 196G/A which causes the 66 Val to be replaced by Met.

BDNF cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BDNF cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BDNF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC
61       ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
121      GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
181      GACACTTTCG AACACATGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
241      GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
301      CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
361      AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
421      TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
481      GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
541      TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
601      AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
661      GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
721      TTGACCATTA AAAGGGGAAG ATAG

 

Quality Control:cDNA sequencing results is available upon request.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"