Mouse / Human BDNF Protein (Native)

Brain-derived neurotrophic factor Protein Datasheet

BDNF Protein Product Information

• Synonym: BDNF
• Protein Construction: A DNA sequence encoding the mature form of mouse BDNF (P21237) (Met1-Arg249) was expressed and purified. Human and Mouse BDNF sequences are identical.
• Source: Mouse
• Expression Host: CHO Stable Cells

BDNF Protein QC Testing

• Purity: > 98% as determined by SDS-PAGE
• Bio-activity: 1. Measured by its ability to bind biotinylated human TrkB-His (Cat:10047-H08H) in functional ELISA.
  2. Measured by its ability to bind Human TrkB-Fch (Cat:10047-H03H) in functional ELISA.
  3. Measured by its ability to bind biotinylated mouse TrkB-His (Cat:50132-M08H) in functional ELISA.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse BDNF consists of 249 amino acids and predicts a molecular mass of 28.1 KDa. It migrates as an approximately 13 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: BDNF protein

BDNF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

BDNF Protein Description

BDNF belongs to the nerve growth factor family. It is induced by cortical neurons, and is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS), also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion.

References

1. Zigova T et al., 1998, Mol Cell Neurosci. 11 (4): 234-45.
2. Acheson A. et al., 1995, Nature 374 (6521): 450-3.
3. Bekinschtein P. et al., 2008, Proc Natl Acad Sci. 105 (7): 2711-6.